Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from .

is a free-living nonpathogenic organism. Like many other bacteria, produces a cell-associated -N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity.

Sustainable Hydrocarbon Oligomer Solvent Systems for Sequestration of Trace Organics from Water.

Low-viscosity poly(α-olefin)s (PAOs) either alone or with functional hydrocarbon oligomer cosolvents are nontoxic, nonvolatile, recyclable solvent systems that effectively and efficiently sequester trace amounts of nonpolar organic compounds such as benzene and halogenated organics from water. More polar compounds including perfluorooctanoic acid and nitrobenzene or water-miscible compounds such as THF and triethylamine can also be sequestered if the PAO phase contains an H-bonding PAO-anchored cosolvent.

Controlled Ring-Opening Metathesis Polymerization with Polyisobutylene-Bound Pyridine-Ligated Ru(II) Catalysts.

This study describes the use of polyisobutylene (PIB) to phase-anchor pyridine ligands that form a phase-separable Grubbs third-generation catalyst. We further show that this complex is useful in ring-opening metathesis polymerization (ROMP) reactions. These PIB-bound pyridine-ligated Grubbs catalysts provide the same benefits of control over polymer chain growth and polydispersity of the product as their low-molecular-weight analogs and reduce Ru leaching in ROMP products from approximately 16% (820 ppm residues) as seen with a similar pyridine-ligated catalyst to a value of approximately 3% (160 ppm residues).

Alternatives for Conventional Alkane Solvents.

The studies described in this paper show that hydrocarbon oligomers are alternatives for low molecular weight alkane solvents. These oligomeric solvents are nontoxic, nonvolatile, and recyclable alternatives to heptane in thermomorphic solvent mixtures that use a polar solvent such as methanol, aqueous ethanol, or DMF or in biphasic mixtures that use acetonitrile. Regardless of which polar solvent is used, hydrocarbon oligomers like poly(α-olefin)s (PAOs) exhibit very low leaching into the polar phase.

Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida.

Pasteurella multocida is a pathogen of veterinary and medical importance. Here, we report the 1.85Å resolution crystal structure of the class C acid phosphatase from this organism (denoted rPmCCAP).

Disposition of gamithromycin in plasma, pulmonary epithelial lining fluid, bronchoalveolar cells, and lung tissue in cattle.

Objective: To determine the disposition of gamithromycin in plasma, pulmonary epithelial lining fluid (PELF), bronchoalveolar lavage (BAL) cells, and lung tissue homogenate in cattle.

Animals: 33 healthy Angus calves approximately 7 to 8 months of age.

Procedures: Calves were randomly assigned to 1 of 11 groups consisting of 3 calves each, which differed with respect to sample collection times.

Expression, purification and crystallization of class C acid phosphatases from Francisella tularensis and Pasteurella multocida.

Class C nonspecific acid phosphatases are bacterial enzymes that are secreted across the cytoplasmic membrane and hydrolyze a variety of phosphomonoesters at acidic pH. These enzymes are of interest for the development of improved vaccines and clinical diagnostic methods. In one case, the category A pathogen Francisella tularensis, the class C phosphatase plays a role in bacterial fitness.

Quantitative HPLC-UV method for the determination of firocoxib from horse and dog plasma.

A sensitive reversed-phase HPLC-UV method was developed for the determination of firocoxib, a novel and highly selective COX-2 inhibitor, in plasma. A 1.0 mL dog or horse plasma sample is mixed with water and passed through a hydrophobic-lipophilic copolymer solid-phase extraction column to isolate firocoxib.