Assessment of in vitro particle dosimetry models at the single cell and particle level by scanning electron microscopy.

Background: Particokinetic models are important to predict the effective cellular dose, which is key to understanding the interactions of particles with biological systems. For the reliable establishment of dose-response curves in, e.g.

Contrast of Backscattered Electron SEM Images of Nanoparticles on Substrates with Complex Structure.

This study is concerned with backscattered electron scanning electron microscopy (BSE SEM) contrast of complex nanoscaled samples which consist of SiO nanoparticles (NPs) deposited on indium-tin-oxide covered bulk SiO and glassy carbon substrates. BSE SEM contrast of NPs is studied as function of the primary electron energy and working distance. Contrast inversions are observed which prevent intuitive interpretation of NP contrast in terms of material contrast.