Is 300 Seconds ACT Safe and Efficient during MiECC Procedures?

Introduction: The recommended minimum activated clotting time (ACT) level for cardiopulmonary bypass (CPB) of 480 seconds originated from investigations with bubble oxygenators and uncoated extracorporeal circulation (ECC) systems. Modern minimal invasive ECC (MiECC) systems are completely closed circuits containing a membrane oxygenator and a tip-to-tip surface coating. We hypothesized that surface coating and the "closed-loop" design allow the MiECC to safely run with lower ACT levels and that an ACT level of 300 seconds can be safely applied without thromboembolic complications.

Shed-blood-separation and cell-saver: an integral Part of MiECC? Shed-blood-separation and its influence on the perioperative inflammatory response during coronary revascularization with minimal invasive extracorporeal circulation systems - a randomized controlled trial.

Objective: The postoperative systemic inflammatory response after cardiopulmonary bypass (CPB) is still an undesirable side-effect after cardiac surgery. It is most likely caused by blood contact with foreign surfaces and by the surgical trauma itself. However, the recirculation of activated shed mediastinal blood is another main cause of blood cell activation and cytokine release.

Assessment of tenderness of aged bovine gluteus medius muscles using Raman spectroscopy.

A portable 671 nm Raman system was evaluated as a rapid and non-destructive device for the assessment of beef tenderness using 175 gluteus medius muscles (99 for calibration, 76 for validation) aged at -1 °C and 7 °C for fourteen days. Raman and shear force (SF) measurements were performed with the aged beef. The samples stored at -1 °C showed on average only slightly increased SF values.

State-of-the-art anterior cruciate ligament tears: A primer for primary care physicians.

The purpose of this article is to provide primary care physicians and other members of the medical community with an updated, general review on the subject of anterior cruciate ligament (ACL) tears. We aim to enhance awareness of these injuries and to prepare those practicing in the primary care setting to address these injuries. Because ACL injuries are quite common, it is very likely that a primary care physician will encounter these injuries and need to address them acutely.

Use of a squatting movement as a clinical marker of function after total knee arthroplasty.

Objective: The aims of this study were to evaluate weight bearing during standing and 30- and 60-degree squats approximately 1 wk and 2 mos after surgery and determine whether weight bearing during squatting could be a better clinical marker than standing for identifying perceived functional limitation approximately 1 wk after surgery. A further objective was to determine whether age, body mass index, and number of outpatient visits over the course of rehabilitation predicted weight bearing during a squat approximately 2 mos after surgery.

Design: The percentage of body weight placed over both limbs during stand and 30- and 60-degree squats in 38 patients (25 women and 13 men) who had primary unilateral knee arthroplasty was determined.

Squatting after primary unilateral total knee arthroplasty: a pilot study examining differences between genders.

Objective: To compare the weight bearing of men and women during standing and squatting after knee replacement surgery for osteoarthritis.

Design: Two-group, single-surgeon study with patients selected consecutively through a sample of convenience. Patients evaluated at first outpatient rehabilitation visit and at discharge from outpatient services.

Closed-chain exercise after simultaneous bilateral knee replacement surgery: a case report.

The purpose of this case report was to present a method for assessing entire lower extremity performance and describe a Medical Exercise Therapy (MET) training program early after simultaneous bilateral total knee replacement (TKR). We also documented perceived function, mobility, and weight-bearing ability during functional tasks. The patient was a 50-year-old male who underwent a simultaneous bilateral procedure and began physical therapy (PT) 16 days postsurgery.

Delaying knee replacement and implications on early postoperative outcomes: a pilot study.

The goal of this study was to compare outcomes during postoperative rehabilitation between individuals who delayed surgery to those who did not delay surgery. Forty-two patients who underwent unilateral total knee replacement (TKR) were categorized into 2 groups: early surgical (n=30, surgery 324 days prior) and late surgical (n=12, surgery >or=325 days from the initial orthopedic office visit). The KSKS, KSFS, SF-12 PC summary, and AROM for knee extension and flexion were assessed preoperatively.

Minimal extracorporeal circulation and off-pump compared to conventional cardiopulmonary bypass in coronary surgery.

Objectives: Although minimal extracorporeal circulation (MECC) and off-pump surgery are equal or better alternatives to conventional cardiopulmonary bypass (CCPB) regarding perioperative morbidity, use of blood and blood products and completeness of revascularization, CCPB is still being used in the majority of coronary artery bypass grafting (CABG) operations.

Methods And Results: We investigated 1472 CABG operations in our center. A total of 1143 CABG operations were performed using CCPB, 220 using MECC and 109 were performed as off-pump coronary artery bypass (OPCAB).

Expression of serologically identified tumor antigens in acute leukemias.

Cancer/testis antigens (CTA) are an expanding family of immunogenic proteins selectively expressed in human neoplasms. As little is known about the expression of serologically identified CTA in leukemias so far, we investigated the expression of 5 CT genes (SSX-1, HOM-MEL-40/SSX-2, HOM-TES-14/SCP-1, SCP-3 and NY-ESO-1) in leukemic blood samples obtained from patients with either acute lymphatic leukemias (ALL) or myelocytic leukemia (AML). RT-PCR-analyses showed no expression of any of the CT-genes in the leukemia samples of 19 patients with AML, whereas frequent expression was found in ALL.

A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing.

We describe here the definition and characterization of antigen CT-8/HOM-TES-85 encoded by a previously unknown gene and identified by serological expression screening using antibodies from a seminoma patient. Intriguingly, the leucine zipper region of CT-8/HOM-TES-85 shows an atypical amphipathy with clusters of hydrophobic residues that is exclusively shared by the N-myc proto-oncogene. CT-8/HOM-TES-85 gene is tightly silenced in normal tissues except for testis.