The binuclear cyclopalladated complex CP2 is targeting ubiquinol-cytochrome c reductase (complex III) of Leishmania amazonensis.

Leishmaniasis is a neglected disease that remains with a limited number of drugs available for chemotherapy and has an increased drug resistance that affects treatment outcomes. Metal-based drugs such as cyclopalladated complex [Pd(dmba)(μ-N)] (CP2), a Leishmania topoisomerase IB inhibitor involved in calcium dysregulation and mitochondrial dysfunction of the parasite, had been an alternative to outline the appearance of chemoresistance. To identify new molecular targets and point out possible resistance mechanisms, a CP2-resistant Leishmania amazonensis (LaR) was selected by stepwise exposure to increasing drug pressure until a line capable of growth in 13.

A role for lysosomal calcium channels in mitigating mitochondrial damage and oxidative stress.

Elevated free fatty acids and oxidative stress may function as pathogenic factors in endothelial dysfunction that is associated with various cardiovascular complications. In recent work, Feng and colleagues report that activation of a lysosomal Ca channel may be a viable option to alleviate oxidative damage by boosting lysosome biogenesis and mitophagy.

On three new species of the earthworm genus Drawida Michaelsen, 1900 (Clitellata: Moniligastridae) from south-western India.

Three new earthworm species of the genus Drawida Michaelsen, 1900, namely D. jatinderi Narayanan, sp. nov.

Impact of Matrix Gel Variations on Primary Culture of Microvascular Endothelial Cell Function.

Objective: The endothelium regulates crucial aspects of vascular function, including hemostasis, vasomotor tone, proliferation, immune cell adhesion, and microvascular permeability. Endothelial cells (ECs), especially in arterioles, are pivotal for flow distribution and peripheral resistance regulation. Investigating vascular endothelium physiology, particularly in microvascular ECs, demands precise isolation and culturing techniques.

Daylight driven vertical migration of mesozooplankton in the Arabian sea and the Bay of Bengal.

Diurnal vertical migration (DVM) of mesozooplankton in the Deep Scattering Layer (DSL) of the Indian seas is poorly studied. This cyclical vertical migration substantially controls the carbon sequestration in the ocean. The present research is a comprehensive examination to analyse the factors affecting the DVM pattern of the zooplankton community in the Arabian Sea (AS) and the Bay of Bengal (BoB).

Catalogue of the moniligastrid earthworms (Clitellata, Moniligastrida, Moniligastridae) of the world.

This study presents a comprehensive catalogue of the moniligastrid earthworms (Moniligastrida, Moniligastridae) of the world. A total of 176 valid species/subspecies belonging to 5 genera are known to exist in this primitive family. Drawida is the speciose genus with 147 species/subspecies.

Redescription of the earthworm Moniligaster gravelyi Stephenson, 1915 (Clitellata, Moniligastridae) based on recent collections from Kerala state, India.

Earthworms of the genus Moniligaster Perrier, 1872 can be distinguished from other genera of the family Moniligastridae by the presence of spermathecal atrial glands. Moniligaster gravelyi Stephenson, 1915 has so far been known only from a single specimen, and it is the only species with supposed single spermathecal atrial gland in the genus. Hence, this species has been a subject to some taxonomic confusion.

Synthesis and commercialization of bioplastics: Organic waste as a sustainable feedstock.

Substituting synthetic plastics with bioplastics, primarily due to their inherent biodegradable properties, represents a highly effective strategy to address the current global issue of plastic waste accumulation in the environment. Advances in bioplastic research have led to the development of materials with improved properties, enabling their use in a wide range of applications in major commercial sectors. Bioplastics are derived from various natural sources such as plants, animals, and microorganisms.

Short-term high-fat diet feeding of mice suppresses catecholamine-stimulated Ca signalling in hepatocytes and intact liver.

Excess consumption of carbohydrates, fat and calories leads to non-alcoholic fatty liver disease (NAFLD) and hepatic insulin resistance; these are major factors in the pathogenesis of type II diabetes. Hormones and catecholamines acting through G-protein coupled receptors (GPCRs) linked to phospholipase C (PLC) and increases in cytosolic Ca ([Ca ] ) regulate many metabolic functions of the liver. In the intact liver, catabolic hormones such as glucagon, catecholamines and vasopressin integrate and synergize to regulate the frequency and extent to which [Ca ] waves propagate across hepatic lobules to control metabolism.

Year-to-year variability of oil pollution along the Eastern Arabian Sea: The impact of COVID-19 imposed lock-downs.

This study investigated the year-to-year variability in the occurrence, abundance and sources of oil spills in the Eastern Arabian Sea (EAS) using sentinel-1 imagery and identified the potential oil spills vulnerable zones. The four consecutive year's data acquired from 2017 to 2020 (March-May) reveal three oil spill hot spot zones. The ship-based oil spills were dominant over zone's-1 (off Gujarat) and 3 (off Karnataka and Kerala), and the oil field based over zone-2 (off Maharashtra).

Cationic helicenes as selective G4 DNA binders and optical probes for cellular imaging.

The important role that G-quadruplex DNA (G4 DNA) structures play in regulating biological processes is becoming widely recognised. These structures have also been proposed to be attractive drug targets. Therefore, there has been significant interest in developing small molecules that can selectively bind to G4 DNA over other topologies.

Role of heavy metal tolerant rhizosphere bacteria in the phytoremediation of Cu and Pb using (Mart.) Solms.

The role of multi-heavy metal tolerant bacteria isolated from the rhizosphere of in the phytoremediation of Cu and Pb under laboratory conditions was investigated. The heavy metal tolerant rhizosphere bacteria were identified as , , , , and Results showed a significant variation in wet weight, Heterotrophic Plate Count (HPC) of the rhizosphere, HPC of water, removal and uptake of Cu and Pb by , either alone or in association with the rhizosphere bacteria. The removal of Cu by in different experimental conditions showed that OTC (Oxytetracycline) untreated with rhizosphere bacteria has maximum removal with 95%, followed by alone with 84%.

Targeting a Novel KRAS Binding Site: Application of One-Component Stapling of Small (5-6-mer) Peptides.

RAS proteins are central in the proliferation of many types of cancer, but a general approach toward the identification of pan-mutant RAS inhibitors has remained unresolved. In this work, we describe the application of a binding pharmacophore identified from analysis of known RAS binding peptides to the design of novel peptides. Using a chemically divergent approach, we generated a library of small stapled peptides from which we identified compounds with weak binding activity.

Receptor-specific Ca oscillation patterns mediated by differential regulation of P2Y purinergic receptors in rat hepatocytes.

Extracellular agonists linked to inositol-1,4,5-trisphosphate (IP) formation elicit cytosolic Ca oscillations in many cell types, but despite a common signaling pathway, distinct agonist-specific Ca spike patterns are observed. Using qPCR, we show that rat hepatocytes express multiple purinergic P2Y and P2X receptors (R). ADP acting through P2Y1R elicits narrow Ca oscillations, whereas UTP acting through P2Y2R elicits broad Ca oscillations, with composite patterns observed for ATP.

New Insights into the Mechanism of Action of the Cyclopalladated Complex (CP2) in : Calcium Dysregulation, Mitochondrial Dysfunction, and Cell Death.

The current treatment of leishmaniasis is based on a few drugs that present several drawbacks, such as high toxicity, difficult administration route, and low efficacy. These disadvantages raise the necessity to develop novel antileishmanial compounds allied with a comprehensive understanding of their mechanisms of action. Here, we elucidate the probable mechanism of action of the antileishmanial binuclear cyclopalladated complex [Pd(dmba)(μ-N)] (CP2) in Leishmania amazonensis.

Fate of MV Wakashio oil spill off Mauritius coast through modelling and remote sensing observations.

This study aims at assessing the fate of MV Wakashio oil spill, and the driving forces responsible for possible environmental consequences of polluted coastal region. GNOME simulations were performed, considering various meteo-oceanographic forcings such as (i) winds and currents, (ii) only winds, and (iii) only winds with different diffusion coefficients, and validated with the satellite images. The results revealed that the simulations performed with 'only winds' reasonably match with the satellite observations, indicating that winds are the primary driving forces.

Three new species and four new species records of earthworms of the genus emMoniligaster/em Perrier, 1872 (Clitellata: Moniligastridae) from Kerala region of the Western Ghats Biodiversity Hotspot, India.

Three new species of Moniligaster Perrier, 1872, namely M. bahli Narayanan Julka, sp. nov.

A dual-channel incoherent broadband cavity-enhanced absorption spectrometer for sensitive atmospheric NO measurements.

We describe and characterize a dual-channel incoherent broadband cavity-enhanced absorption spectrometer (DC-IBBCEAS) for the sensitive measurements of NOx: the sum of nitrogen monoxide (NO) and nitrogen dioxide (NO2) in the atmosphere. The instrument employs two 1 m long optical cavities, with air being extracted from a common sampling line. The first channel (cavity-1) spans 340-380 nm and the second channel (cavity-2) spans the 405-460 nm spectral range, both measuring NO2 simultaneously.

A Tale of two receptors.

Calcium (Ca) oscillations in hepatocytes have a wide dynamic range. In particular, recent experimental evidence shows that agonist stimulation of the P2Y family of receptors leads to qualitatively diverse Ca oscillations. We present a new model of Ca oscillations in hepatocytes based on these experiments to investigate the mechanisms controlling P2Y-activated Ca oscillations.

Ethanol Disrupts Hormone-Induced Calcium Signaling in Liver.

Receptor-coupled phospholipase C (PLC) is an important target for the actions of ethanol. In the ex vivo perfused rat liver, concentrations of ethanol >100 mM were required to induce a rise in cytosolic calcium (Ca) suggesting that these responses may only occur after binge ethanol consumption. Conversely, pharmacologically achievable concentrations of ethanol (≤30 mM) decreased the frequency and magnitude of hormone-stimulated cytosolic and nuclear Ca oscillations and the parallel translocation of protein kinase C-β to the membrane.