The potential of SNP-based PCR-RFLP capillary electrophoresis analysis to authenticate and detect admixtures of Mediterranean olive oils.

Authentication and traceability of extra virgin olive oil is a challenging research task due to the complexity of fraudulent practices. In this context, the monovarietal olive oils of Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) require new tests and cutting edge analytical technologies to detect mislabeling and misleading origin. Toward this direction, DNA-based technologies could serve as a complementary to the analytical techniques assay.

454 Pyrosequencing of Olive (Olea europaea L.) Transcriptome in Response to Salinity.

Olive (Olea europaea L.) is one of the most important crops in the Mediterranean region. The expansion of cultivation in areas irrigated with low quality and saline water has negative effects on growth and productivity however the investigation of the molecular basis of salt tolerance in olive trees has been only recently initiated.

A SNP-based PCR-RFLP capillary electrophoresis analysis for the identification of the varietal origin of olive oils.

Authenticity and traceability of high quality monovarietal extra virgin olive oils is a major concern for markets and consumers. Although analytical chemistry techniques are widely used to satisfy these needs recently developed DNA-based methods can serve as complementary approaches. A SNP database comprising 10 Greek olive varieties was constructed and five SNPs, residing in restriction sites, were selected for the development of a PCR-RFLP capillary electrophoresis method to discriminate these varieties using leaf DNA as template.

Characterization of two carnation petal prolyl 4 hydroxylases.

Prolyl 4-hydroxylases (P4Hs) catalyze the proline hydroxylation, a major post-translational modification, of hydroxyproline-rich glycoproteins. Two carnation petal P4H cDNAs, (Dianthus caryophyllus prolyl 4-hydroxylase) DcP4H1 and DcP4H2, were identified and characterized at the gene expression and biochemical level in order to investigate their role in flower senescence. Both mRNAs showed similar patterns of expression with stable transcript abundance during senescence progression and differential tissue-specific expression with DcP4H1 and DcP4H2 strongly expressed in ovaries and stems, respectively.

Involvement of Arabidopsis prolyl 4 hydroxylases in hypoxia, anoxia and mechanical wounding.

Arabidopsis prolyl 4 hydroxylases (P4Hs) catalyze an important post-translational modification in plants, though the only information on their patterns of expression is solely based on Arabidopsis microarray analysis data. In addition, the expression patterns of plants P4Hs in response to hypoxia, anoxia and other abiotic stresses such as mechanical wounding have never been studied extensively, despite their central role in hypoxic response of several other organisms through the regulation of stability of the HIF-1alpha transcription factor, the global regulator of hypoxic response. The 13 putative Arabidopsis P4Hs are low abundance transcripts with differential patterns of expression in response to two hypoxic, 1.