Cutaneous lesions in sarcoidosis are characterized by epithelioid nodules surrounded by lymphocytes. The B- or T-cell subpopulation was identified by quantitative immunological methods on freed cells and immunocytochemical labelling on tissue sections. Most of the liberated cells formed spontaneous rosettes with sheep erythrocytes and were devoid of membrane immunoglobulins.
View Article and Find Full Text PDFImmunological characterization of cells in the malignant epidermotropic lymphomas requires techniques which define the lymphocytic nature of the cells, and for the lymphocytes techniques which demonstrate subpopulations of T or B cells. The results obtained using such methods in patients with cutaneous lymphomas are reported. The predominantly thymodependent nature of the cells of epidermotropic lymphomas is confirmed and the existence of a thymodependent non-epidermotropic cutaneous lymphoma is demonstrated.
View Article and Find Full Text PDFAnn Dermatol Venereol
January 1978
A 50 year old man with actinic reticuloid developed a diffuse malignant lymphoma 7 years after the beginning of the dermatosis. Biopsy of lymph node showed a polymorphic proliferation with lymphocytes, numerous reticular cells and several Sternberg-like cells. An ultrastructural study showed an indifferentiate lymphocyte proliferation and numerous malignant reticular cells without the Sternberg cell picture.
View Article and Find Full Text PDFThe malignant cutaneous lymphomas come into the category of hematodermias but can equally be considered as an abnormality of the immune system. Having described the methods used in the immunocytological investigation of 21 lymphomas and 3 pseudolymphomas, the authors expound their classification of malignant cutaneous lymphomas before stating the results obtained in immunocytological studies in each of the groups. They show how formal separation between epidermtropic malignant cutaneous lymphomas and non-epidermotropic malignant cutaneous lymphomas can be confirmed by immunocytological and ultrastructural facts.
View Article and Find Full Text PDFActa Derm Venereol
February 1979
Progressive depigmentation of the coat has been observed in C3H mice thymectomized at birth and reared in a germ-free environment. The origin of the pigmentary anomalies is discussed. It is probable that a causative relationship exists between thymectomy and the acquired depigmentation observed.
View Article and Find Full Text PDFAnn Dermatol Venereol
October 1977
Ann Dermatol Venereol
October 1977
The specific humoral and cellular immunity of 22 patients with multiple or recurring warts was studied. After repeated intradermal tests, using an inactivated, purified viral antigen, the responses obtained could be classed into two groups. The first group (10 patients) was characterized immunologically by the acquisition of a specific cellular immunity and the appearance of circulating IgG antibodies, and clinically by a total regression of resolution of the warts two to three weeks after the final intradermal test.
View Article and Find Full Text PDFArch Dermatol Res (1975)
September 1977
Arch Dermatol Res (1975)
September 1977
A simple technique using immunoperoxidase-labelled antibodies is reported for the ultrastructural localization of fixed immunoglobulins in tissue lesions. This technique allows the immunolabeling of frozen sections of 15 mu mounted on glass slides. These sections are then closed over for ultrastructural examination, using an Epon-filled gelatin capsule, and then returned to the glass slide.
View Article and Find Full Text PDFClin Immunol Immunopathol
September 1977
Arch Dermatol Res (1975)
August 1977
The authors studied by electron microscope, the sun-exposed skin of the back of the hand from three heavily hemodialysed patients with a porphyria cutanea tarda-like bullous skin disease. The vascular impairment, like that of PCT, closely resembles that seen during medicamentous phototoxic processes. The connective tissue is infiltrated by large granulo-filamentous masses and the fibroblasts are secretory in appearance.
View Article and Find Full Text PDFArch Dermatol Res (1975)
August 1977
In cutaneous sarcoidosis, mononuclear cells of granuloma have been freed and characterized by immunological tests. Of these cells, 84% of them were found to be T dependent by the E rosette test. A similar value (79%) was obtained in the stroma of delayed hypersensitivity reaction, whose immunological functions are well known.
View Article and Find Full Text PDFPurified human papilloma virus (HPV) was prepared from plantar warts, inactivated by formalin, adjusted to 10 particles per 0·1 ml and used in intradermal tests (IDT) on 120 patients having warts at that time, or having had warts in the past, together with sixty-three controls. Antibodies were evaluated in the sera from most of the patients by the indirect immunofluorescence (IF) test before and after the skin tests. The results showed a specific delayed-hypersensitivity reaction (DHR) to HPV, especially in patients with `regressing' or `past' warts (76%).
View Article and Find Full Text PDFThe identification of mononuclear cells extracted from cutaneous tumours (basal cell carcinoma, squamous cell carcinoma and superficial, spreading melanoma) has been investigated. The relative numbers of T cells and B cells have been determined using the E-rosette test and the EAC-rosette test. The results have been compared to those of delayed hypersensitivity type reactions.
View Article and Find Full Text PDFAn immunocytochemical technique has been developed for the identification in situ of immunoglobulin-producing cells in tissues fixed in Bouin's solution and embedded in paraffin. Technical details are discussed as well as the application of the technique to the study of plasma cells in the inflammatory infiltrate around cutaneous tumors. Preliminary results have been obtained with basal cell epitheliomas, squamous cell carcinomas, and malignant melanomas.
View Article and Find Full Text PDFAnn Dermatol Venereol
May 1977
A 37 years old woman presented with scleroderma en coup de sabre of the head with chronic homolateral ocular hypertony. Skin disorders appeared in the childhood and extended slowly until the age of 20. Glaucoma, found 5 years earlier, was unimproved by medical treatment; the opposite eye was normal.
View Article and Find Full Text PDFAnn Dermatol Venereol
February 1977
The authors report 10 cases of selected Gougerot-Ruiter's disease and 13 cases of various vasculitis. Direct immunofluorescence studies were performed on the skin lesions and the results were correlated to various biological parameters such as dosage of serum IgA, IgG and IgM, antinuclear antibodies, Australia antigen. Complement (beta-1-C/beta-1-A) deposition was found in 9 out of 10 cases of Gougerot-Ruiter's disease as opposed to immunoglobulin deposition found only in 4 out of 10 cases.
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