[Cutaneous sarcoid granuloma: cytoimmunological study of the lymphocytes (freed cells and tissue sections) (author's transl)].

Cutaneous lesions in sarcoidosis are characterized by epithelioid nodules surrounded by lymphocytes. The B- or T-cell subpopulation was identified by quantitative immunological methods on freed cells and immunocytochemical labelling on tissue sections. Most of the liberated cells formed spontaneous rosettes with sheep erythrocytes and were devoid of membrane immunoglobulins.

[Immunological characterization of malignant epidermotropic lymphoma cells in cutaneous infiltrates (author's transl)].

Immunological characterization of cells in the malignant epidermotropic lymphomas requires techniques which define the lymphocytic nature of the cells, and for the lymphocytes techniques which demonstrate subpopulations of T or B cells. The results obtained using such methods in patients with cutaneous lymphomas are reported. The predominantly thymodependent nature of the cells of epidermotropic lymphomas is confirmed and the existence of a thymodependent non-epidermotropic cutaneous lymphoma is demonstrated.

[Generalized lymph node sarcoma during course of an actinic reticuloid (author's transl)].

A 50 year old man with actinic reticuloid developed a diffuse malignant lymphoma 7 years after the beginning of the dermatosis. Biopsy of lymph node showed a polymorphic proliferation with lymphocytes, numerous reticular cells and several Sternberg-like cells. An ultrastructural study showed an indifferentiate lymphocyte proliferation and numerous malignant reticular cells without the Sternberg cell picture.

[Immunocytological study of cutaneous malignant lymphomas. Classification (author's transl)].

The malignant cutaneous lymphomas come into the category of hematodermias but can equally be considered as an abnormality of the immune system. Having described the methods used in the immunocytological investigation of 21 lymphomas and 3 pseudolymphomas, the authors expound their classification of malignant cutaneous lymphomas before stating the results obtained in immunocytological studies in each of the groups. They show how formal separation between epidermtropic malignant cutaneous lymphomas and non-epidermotropic malignant cutaneous lymphomas can be confirmed by immunocytological and ultrastructural facts.

Progressive depigmentation of the coat of C3H mice after neonatal thymectomy.

Progressive depigmentation of the coat has been observed in C3H mice thymectomized at birth and reared in a germ-free environment. The origin of the pigmentary anomalies is discussed. It is probable that a causative relationship exists between thymectomy and the acquired depigmentation observed.

Specific immunity in patients suffering from recurring warts before and after repetitive intradermal tests with human papilloma virus.

The specific humoral and cellular immunity of 22 patients with multiple or recurring warts was studied. After repeated intradermal tests, using an inactivated, purified viral antigen, the responses obtained could be classed into two groups. The first group (10 patients) was characterized immunologically by the acquisition of a specific cellular immunity and the appearance of circulating IgG antibodies, and clinically by a total regression of resolution of the warts two to three weeks after the final intradermal test.

A simple technique for immunoelectronmicroscopic localization of Ig on tissue sections with special reference to cutaneous pathology.

A simple technique using immunoperoxidase-labelled antibodies is reported for the ultrastructural localization of fixed immunoglobulins in tissue lesions. This technique allows the immunolabeling of frozen sections of 15 mu mounted on glass slides. These sections are then closed over for ultrastructural examination, using an Epon-filled gelatin capsule, and then returned to the glass slide.

Porphyria cutanea tarda-like dermatosis by hemodialysis. Ultrastructural study of exposed skin.

The authors studied by electron microscope, the sun-exposed skin of the back of the hand from three heavily hemodialysed patients with a porphyria cutanea tarda-like bullous skin disease. The vascular impairment, like that of PCT, closely resembles that seen during medicamentous phototoxic processes. The connective tissue is infiltrated by large granulo-filamentous masses and the fibroblasts are secretory in appearance.

Evidence for a T-cell reaction in the cutaneous sarcoid granuloma.

In cutaneous sarcoidosis, mononuclear cells of granuloma have been freed and characterized by immunological tests. Of these cells, 84% of them were found to be T dependent by the E rosette test. A similar value (79%) was obtained in the stroma of delayed hypersensitivity reaction, whose immunological functions are well known.

Comparative study of delayed hypersensitivity skin reactions and antibodies to human papilloma virus (HPV).

Purified human papilloma virus (HPV) was prepared from plantar warts, inactivated by formalin, adjusted to 10 particles per 0·1 ml and used in intradermal tests (IDT) on 120 patients having warts at that time, or having had warts in the past, together with sixty-three controls. Antibodies were evaluated in the sera from most of the patients by the indirect immunofluorescence (IF) test before and after the skin tests. The results showed a specific delayed-hypersensitivity reaction (DHR) to HPV, especially in patients with `regressing' or `past' warts (76%).

Characterization of mononuclear cells in the inflammatory infiltrates of cutaneous tumours.

The identification of mononuclear cells extracted from cutaneous tumours (basal cell carcinoma, squamous cell carcinoma and superficial, spreading melanoma) has been investigated. The relative numbers of T cells and B cells have been determined using the E-rosette test and the EAC-rosette test. The results have been compared to those of delayed hypersensitivity type reactions.

Immunoglobulin-producing cells in the inflammatory infiltrates of cutaneous tumors. Immunocytologic identification in situ.

An immunocytochemical technique has been developed for the identification in situ of immunoglobulin-producing cells in tissues fixed in Bouin's solution and embedded in paraffin. Technical details are discussed as well as the application of the technique to the study of plasma cells in the inflammatory infiltrate around cutaneous tumors. Preliminary results have been obtained with basal cell epitheliomas, squamous cell carcinomas, and malignant melanomas.

[Scleroderma en coup de sabre with homolateral chronic glaucoma (author's transl)].

A 37 years old woman presented with scleroderma en coup de sabre of the head with chronic homolateral ocular hypertony. Skin disorders appeared in the childhood and extended slowly until the age of 20. Glaucoma, found 5 years earlier, was unimproved by medical treatment; the opposite eye was normal.

[Immunopathological study of Gougerot-Ruiter's vasculitis (author's transl)].

The authors report 10 cases of selected Gougerot-Ruiter's disease and 13 cases of various vasculitis. Direct immunofluorescence studies were performed on the skin lesions and the results were correlated to various biological parameters such as dosage of serum IgA, IgG and IgM, antinuclear antibodies, Australia antigen. Complement (beta-1-C/beta-1-A) deposition was found in 9 out of 10 cases of Gougerot-Ruiter's disease as opposed to immunoglobulin deposition found only in 4 out of 10 cases.