A self-powered ingestible wireless biosensing system for real-time in situ monitoring of gastrointestinal tract metabolites.

Information related to the diverse and dynamic metabolite composition of the small intestine is crucial for the diagnosis and treatment of various diseases. However, our current understanding of the physiochemical dynamics of metabolic processes within the small intestine is limited due to the lack of in situ access to the intestinal environment. Here, we report a demonstration of a battery-free ingestible biosensing system for monitoring metabolites in the small intestine.

Disposable Microchamber with a Microfluidic Paper-Based Lid for Generation and Membrane Separation of SO Gas Employing an In Situ Electrochemical Gas Sensor for Quantifying Sulfite in Wine.

This work presents a fully disposable microchamber for gas generation of a sample solution. The microchamber consists of a cylindrical well-reactor and a paper-based microfluidic lid (μFluidic lid), which also serves as the reagent loading and dispensing unit. The base of the reactor consists of a hydrophobic membrane covering an in-house graphene electrochemical gas sensor.

Non-Invasive Sweat-Based Tracking of L-Dopa Pharmacokinetic Profiles Following an Oral Tablet Administration.

Levodopa (L-Dopa) is the "gold-standard" medication for symptomatic therapy of Parkinson disease (PD). However, L-Dopa long-term use is associated with the development of motor and non-motor complications, primarily due to its fluctuating plasma levels in combination with the disease progression. Herein, we present the first example of individualized therapeutic drug monitoring for subjects upon intake of standard L-Dopa oral pill, centered on dynamic tracking of the drug concentration in naturally secreted fingertip sweat.

Gold leaf electrochemical sensors: applications and nanostructure modification.

This work presents the first planar three-electrode electrochemical sensor comprising local gold leaf as the working electrode and printed, or hand-drawn, counter and reference electrodes, respectively. The gold leaf was mounted on a polyvinyl chloride (PVC) adhesive sheet (15 mm × 30 mm) and covered with a second PVC sheet printed with the counter and reference electrodes. This sheet has a 3 mm circle and a 2 mm × 3 mm rectangle removed to expose the gold electrode area and electrical contacts, respectively.

An epidermal patch for the simultaneous monitoring of haemodynamic and metabolic biomarkers.

Monitoring the effects of daily activities on the physiological responses of the body calls for wearable devices that can simultaneously track metabolic and haemodynamic parameters. Here we describe a non-invasive skin-worn device for the simultaneous monitoring of blood pressure and heart rate via ultrasonic transducers and of multiple biomarkers via electrochemical sensors. We optimized the integrated device so that it provides mechanical resiliency and flexibility while conforming to curved skin surfaces, and to ensure reliable sensing of glucose in interstitial fluid and of lactate, caffeine and alcohol in sweat, without crosstalk between the individual sensors.

Simple Flow-Based System with an In-Line Membrane Gas-liquid Separation Unit and a Contactless Conductivity Detector for the Direct Determination of Sulfite in Clear and Turbid Food Samples.

This study presents a simple flow-based system for the determination of the preservative agent sulfite in food and beverages. The standard method of conversion of sulfite ions into SO gas by acidification is employed to separate the sulfite from sample matrices. The sample is aspirated into a donor stream of sulfuric acid.

Contactless conductivity sensor employing moist paper as absorbent for in-situ detection of generated carbon dioxide gas.

This work presents an unconventional use of capacitively coupled contactless conductivity detector (C4D) for detection of gas absorption by moist paper with potential application for chemical analysis. To be suitable for measuring conductivity of moist paper absorbent, the C4D sensor was therefore designed in planar configuration. A layer of dry filter paper, only 20 mm × 25 mm in size, was placed on the C4D sensor and the device installed inside a specifically designed vaporization chamber.

Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood.

This work presents a novel development that exploits the concept of in-situ gas-separation together with a specific enzymatic colorimetric detection to produce a portable biosensor called "Blood Alcohol Micro-pad" for direct quantitation of ethanol in whole blood. The thin square device (25 mm × 25 mm × 1.8 mm) comprises two layers of patterned filter paper held together with a double-sided mounting tape with an 8-mm circular hole (the headspace).

Contactless conductivity detector from printed circuit board for paper-based analytical systems.

This work presents a capacitively coupled contactless conductivity detector (CD) etched out from a printed circuit board (PCB) as potential sensor for paper-based analytical systems. Two lines of any desirable pattern forming 35-μm thick planar copper electrodes were produced on a PCB plate (40 mm × 60 mm) by photolithography. The final PCB plate was covered with polypropylene film to serve as the insulating layer for the CD detector.

Miniaturized Potentiometric Titration for Improving Portability and Accuracy in the Determination of Total Acid in Squeezed Fruit Juice.

To determine the total acidity in freshly squeezed fruit juice, we miniaturized the potentiometric titrations and achieved better accuracy compared with titrations from a conventional pH probe. The improvement was the result of a higher jump in pH at the endpoint due to a reduction in the dilutions of both the titrand and titrant. A conventional pH probe requires more than 50 mL of titrand, which can lead to a 25000-fold dilution of the titrant when adding the titrant at 2 µL intervals.

Simple and green method for direct quantification of hypochlorite in household bleach with membraneless gas-separation microfluidic paper-based analytical device.

This work presents development of a microfluidic paper-based analytical device (µPAD) for direct determination of hypochlorite in household bleach. The recent design of a membraneless gas-separation microfluidic paper-based analytical device (MBL-GS µPAD) was employed to fabricate the hypochlorite-µPAD. Chlorine gas is generated in the µPAD via acidification of an aliquot of sample loaded on to the donor reservoir located at the bottom layer of the μPAD.