Comparative Evaluation of the Fracture Strength of Pulpotomized Primary Molars: An Study.

Purpose: This study evaluates the fracture strength of pulpotomized primary molars restored with amalgam, miracle mix, cermet, resin-modified glass ionomer cement, and nanocomposites.

Materials And Methods: Fifty primary first and second molars were collected for this study. All the teeth were randomly divided into five groups ( = 10).

Differential requirements of singleplex and multiplex recombineering of large DNA constructs.

Recombineering is an in vivo genetic engineering technique involving homologous recombination mediated by phage recombination proteins. The use of recombineering methodology is not limited by size and sequence constraints and therefore has enabled the streamlined construction of bacterial strains and multi-component plasmids. Recombineering applications commonly utilize singleplex strategies and the parameters are extensively tested.

Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli.

The lambda phage Red recombination system can mediate efficient homologous recombination in Escherichia coli, which is the basis of the DNA engineering technique termed recombineering. Red mediated insertion of DNA requires DNA replication, involves a single-stranded DNA intermediate and is more efficient on the lagging strand of the replication fork. Lagging strand recombination has also been postulated to explain the Red mediated repair of gapped plasmids by an Okazaki fragment gap filling model.

Subcloning plus insertion (SPI)--a novel recombineering method for the rapid construction of gene targeting vectors.

Gene targeting refers to the precise modification of a genetic locus using homologous recombination. The generation of novel cell lines and transgenic mouse models using this method necessitates the construction of a 'targeting' vector, which contains homologous DNA sequences to the target gene, and has for many years been a limiting step in the process. Vector construction can be performed in vivo in Escherichia coli cells using homologous recombination mediated by phage recombinases using a technique termed recombineering.

MicroRNA expression profiling in patients with lamin A/C-associated muscular dystrophy.

Mutations in the lamin A/C gene (LMNA) cause several disorders referred to as laminopathies, which include premature aging syndromes, lipodystrophy, and striated muscle disorders. There is evidence that lamin A/C plays a role in gene expression. MicroRNAs (miRNAs) are short noncoding RNAs regulating mRNAs involved in various biological processes, including the pathophysiology of striated muscles.

Regional odontodysplasia.

Regional odontodysplasia (ROD) is a rare developmental anomaly involving both mesodermal and ectodermal components in a group of contiguous teeth. It affects the primary and permanent dentitions in the maxilla and the mandible or both, however, the maxilla is frequently involved. Although the etiology of the ROD is uncertain, it has been suggested that numerous other factors play a role.

Thermosensitive transparent semi-interpenetrating polymer networks for wound dressing and cell adhesion control.

Thermosensitive, transparent, and flexible semi-interpenetrating polymer networks (semi-IPNs) composed of segmented polyurethane urea/poly(N-isopropylacrylamide) (SPUU/ PNiPAAm) were new class of materials, which holds promise for its potential use as wound dressings. A series of semi-IPNs, obtained via thermal initiated polymerization of NiPAAm, were characterized by infrared spectroscopy (IR), nuclear magnetic resonance (NMR), dynamic viscoelastic measurements, wide-angle X-ray diffraction (WAXD), and mechanical properties. The resulting semi-IPNs were also investigated for their dynamic water contact angles, thermodynamic interaction parameters, in vitro drug release, and cell adhesion and detachment.

Swelling behavior and controlled release of theophylline and sulfamethoxazole drugs in beta-lactoglobulin protein gels obtained by phase separation in water/ethanol mixture.

Physically cross-linked beta-lactoglobulin (BLG) protein gels containing theophylline and sulfamethoxazole low molecular weight drugs were prepared in 50% ethanol solution at pH 8 and two protein concentrations (6 and 7% (w/v)). Swelling behavior of cylindrical gels showed that, irrespective of the hydrated or dehydrated state of the gel, the rate of swelling was the highest in water. When the gels were exposed to water, they first showed a swelling phase in which their weight increased 3 and 30 times for hydrated and dehydrated gels, respectively, due to absorption of water, followed by a dissolution phase.

In situ polymerizable polyethyleneglycol containing polyesterpolyol acrylates for tissue sealant applications.

Polyesterpolyol macromers were prepared with succinic acid and polyethylene glycols (PEG) of different molecular weights. The resulting polyols were acrylated to render them photo-cross-linkable. They could be very rapidly cross-linked into non-tacky films with long-wavelength UV radiation.

Asymmetric Synthesis and Properties of Sulfinimines (Thiooxime S-Oxides).

Enantiomerically pure sulfinimines (thiooxime S-oxides 10), important building blocks in the asymmetric synthesis of amine derivatives, are prepared in good to excellent yields in one step from aromatic, heteroaromatic, and aliphatic aldehydes. This protocol involves treating commercially available (R)- or (S)-menthyl p-toluenesufinate (Andersen reagent 4) with LiHMDS, followed by the aldehyde, affording (E)-10 exclusively. The sulfinimines 10 are formed via a Peterson-type olefination reaction of silylsulfinamide anion 13 with the aldehyde.