Effects of serotonin and ketanserin on the functional morphology of chick down feather bulbi in vitro.

Chick feather bulbi cultured in vitro showed an increased DNA synthesis and a delayed keratinization after treatment with ketanserin, a serotonin2 antagonist with wound-healing properties. In contrast, serotonin stimulates keratinization of the keratinocytes in the bulbus.

Direct effects of serotonin, and 5-HT2, alpha 1 and H1 receptor-antagonists on embryonic chick skin in vitro: a morphological and functional study.

Ketanserin (K), a 5-HT2, alpha 1 and H1 receptor antagonist, at a concentration of 5 micrograms/ml inhibits keratinisation, but increases DNA-, collagen and glycosaminoglycan (GAG) synthesis in embryonic chick skin in vitro. On the other hand, serotonin (S) at a concentration of 10 micrograms/ml stimulates keratinisation and does not enhance DNA, collagen and GAG synthesis. 5 nM doses of ketanserin and serotonin give analogous results.

Dose- and time-dependent radiation inhibition of RNA and glycosaminoglycan synthesis in embryonic cartilage: an in vitro study.

Radiation effects on the RNA and glycosaminoglycan (GAG) synthesis of embryonic cartilaginous tibiae were studied in vitro during a 4- or 7-day culture period. Before being cultured, tibiae received single radiation doses of 20, 50 or 100 Gy. The counterparts served as sham-irradiated controls.

Direct effects of serotonin and ketanserin on the functional morphology of embryonic chick skin in vitro.

Different organotypical culture methods are used to test direct effects of serotonin and ketanserin, a S2, alpha 1, and H1 receptor antagonist in vascular tissue, on fibroblasts and epidermal cells of embryonic chick skin in vitro. From light microscopic and electron microscopic analyses, we learn that serotonin enhances keratinization and differentiation, whereas ketanserin reduces differentiation in comparison to the control cultures. Incorporation data of fragments cultured with [3H]thymidine show that ketanserin, within a dose range from 0.

The effects of ionizing radiation on the glycosaminoglycan synthesis in embryonic cartilage in vitro.

The effects of ionizing radiation on the glycosaminoglycan synthesis of cartilaginous tibiae of 6.5 day old chick embryos have been studied in vitro over a 3 day period. As a parameter for the glycosaminoglycan synthesis, the incorporation of (3)H-glucosamine is evaluated by liquid scintillation counting.

Growth inhibition of cartilaginous embryonic chick tibiae by ionizing radiation: an in vitro study.

The radiation effect on the longitudinal growth of cartilaginous tibiae of 6,5 day old chick embryos is studied in vitro. After irradiation length is measured daily during the 3 days culture period and incorporation of (3)H-thymidine, (3)H-uridine or (3)H-proline is sampled by liquid scintillation counting. Selective tibiae are videorecorded during the incubation period.

Effects of ionizing radiation on the metabolism and longitudinal growth of cartilaginous embryonic chick tibiae in vitro.

The effect of ionizing radiation on the metabolism and longitudinal growth of cartilaginous tibiae of 6.5-day-old chick embryos was studied in vitro over a 3-day period. Before being cultured, tibiae received absorbed doses of 2 to 200 Gy.

Conversion factor f for X-ray beam qualities, specified by peak tube potential and HVL value.

Conversion factors of exposure to absorbed dose f in compact bone, muscle, water and adipose tissue are deduced from the measured photon fluence spectra of x-ray beams with potentials from 50 to 250 kV. The results show that accurate determination of f requires beam quality specification not only by the HVL value but also by a second parameter, e.g.