New dihydrophenanthrenes from .

Three new dihydrophenanthrenes were isolated from the whole plant of collected in Vietnam. These compounds were identified as 2,3,5-trihydroxy-9,10-dihydrophenanthrene (), 2-methoxy-5-hydroxy-9,10-dihydrophenanthrene 3-O--D-glucopyranoside (), and 2,5-dihydroxy-9,10-dihydrophenanthrene 3-O--D-glucopyranoside (). Their structures were determined by HR-ESI-MS and 1D, 2D NMR spectroscopy.

GPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT.

Background: Clear cell renal cell carcinoma (ccRCC), with its hallmark phenotype of high cytosolic lipid content, is considered a metabolic cancer. Despite the implication of this lipid-rich phenotype in ccRCC tumorigenesis, the roles and regulators of de novo lipid synthesis (DNL) in ccRCC remain largely unexplained.

Methods: Our bioinformatic screening focused on ccRCC-lipid phenotypes identified glutathione peroxidase 8 (GPX8), as a clinically relevant upstream regulator of DNL.

Non-bioenergetic roles of mitochondrial GPD2 promote tumor progression.

Despite growing evidence for mitochondria's involvement in cancer, the roles of specific metabolic components outside the respiratory complex have been little explored. We conducted metabolomic studies on mitochondrial DNA (mtDNA)-deficient (ρ0) cancer cells with lower proliferation rates to clarify the undefined roles of mitochondria in cancer growth. Despite extensive metabolic downregulation, ρ0 cells exhibited high glycerol-3-phosphate (G3P) level, due to low activity of mitochondrial glycerol-3-phosphate dehydrogenase (GPD2).

New phenolics from (L.) DC.

Reinvestigation of a methanol extract of afforded a new 3- hydroxyisoflavanone, 3,5,7,2',4'-pentahydroxyisoflavanone (), two new monoaryl glucosides, 3,4-dimethoxyphenyl 1--(6'--acetyl)--D-glucopyranoside ( and 3,4,5-trimethoxyphenyl 1--(6'--acetyl)--D-glucopyranoside (), in addition to three known compounds, 3'--methylorobol (), robusflavone B (), and apigenin (). The structural elucidation of these compounds was achieved by analyses of their spectroscopic data (HR-ESI-MS, 1 D- and 2 D-NMR) and acidic hydrolysis. The extracts and compounds - exhibited weak or no cytotoxic activity against KB, HepG2, Lu and MCF7 cell lines.

Igalan from Inula helenium (L.) suppresses the atopic dermatitis-like response in stimulated HaCaT keratinocytes via JAK/STAT3 signaling.

Objective: This study aimed to evaluate the protective effect of igalan, a sesquiterpene lactone isolated from Inula helenium (L.), on inhibiting inflammation, regulating the epidermal differentiation gene expression, and reactive oxygen species scavenging in atopic dermatitis (AD)-like inflammatory keratinocytes.

Methods: HaCaT human keratinocytes were treated with igalan at indicated concentrations before being activated by a combination of TNF-α and IFN-γ or IL-4 representative for T-helper 1 and T-helper 2 cell cytokines, which are associated with AD pathogenesis.

New phenolics from .

Phytochemical investigation and chromatographic separation of extracts from the aerial parts of (L.) DC. (synonym Lam.

A new isoflavanone from .

A new isoflavanone, (3)-5,7-dihydroxy-2',3',4'-trimethoxy-6,5'-diprenylisoflavanone () and eight known compounds including five flavones (), two triterpenes () and a steroid () were isolated from the whole plant of (Leguminosae). The structure of was elucidated by detailed spectroscopic means including IR, HR-ESI-MS, 1D and 2D NMR, and CD data. Compounds were evaluated for their cytotoxicity against four human cancer cell lines KB (mouth epidermal carcinoma), HepG2 (hepatocellular carcinoma), Lu (lung carcinoma) and MCF7 (breast carcinoma).

Anticancer Activity of Methanol Extract against Human Hepatocellular Carcinoma Cells.

This research aimed to investigate the cytotoxicity of methanol extract of leaf (YLE) against a human hepatocellular carcinoma cell line (HepG2). This plant is currently used as a traditional herbal remedy in the treatment of liver diseases in some rural parts of Myanmar. : The cytotoxic activity of the plant extract against the cancerous cell line was assessed using an MTT assay.

A new oleanane triterpene from the leaves of .

Repeated column chromatography of the n-hexane extract of leaves (Moraceae) led to isolation of a new oleanane triterpene, 3-hydroxy-11-oxo-olean-12-enyl-3-stearate () in addition to three known compounds, taraxerol (), 3-acetoxy-11-methoxy-12-ursene () and 3-acetoxy-11-hydroxy-12-ursene (). Their structures were elucidated by spectroscopic methods and by comparison with data reported in the literatures.

Leaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells.

L., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity.

Anti-proliferative diterpenes from .

A new diterpene, cassipouryl hexadecanoate (), in addition to the cassipourol () and four terpenes () were isolated from the twigs and leaves of (Blume) de Laub. The structures of the two monocyclic diterpenes (, ), were elucidated on the basic of 1D and 2D NMR spectroscopic data and compared with the literature. These two monocyclic diterpenes (, ) were tested for their anti-proliferative activity on acute myeloid leukemia (OCI-AML) cells.

Chemical constituents of Oldenlandia pinifolia and their antiproliferative activities.

This study describes the chemical constituents of Oldenlandia pinifolia (Wall. Ex G. Don) Kuntze (synonym Hedyotis pinifolia Wall.

Chemical constituents from the leaves of Pinus dalatensis Ferré.

A phytochemical study of n-hexane and ethyl acetate extracts of Pinus dalatensis Ferré leaves led to the isolation of 11 compounds, including one caryolane sesquiterpenoid (1), five labdane diterpenoids (2, 3, 4, 5, 6), one serratane triterpenoid (7), one diacylated flavonoid glucoside (8), one stilbenoid (9) and two sterols (10, 11). The structural characterisation of the isolated compounds was elucidated by spectroscopic data and comparison with the literature report on the chemical constituents from Pinus dalatensis Ferré. Futhermore, three compounds 1, 4 and 6 were obtained for the first time from the genus Pinus.

In vivo anticancer activity of maesopsin 4-O-β-glucoside isolated from leaves of Artocarpus tonkinensis A. Chev. Ex Gagnep.

Objective: To investigate the antitumor effect of maesopsin 4-O-β-glucoside (TAT2) isolated from the leaves of Artocarpus tonkinensis (A. tonkinensis) A. Chev.

Preparation of fungal conidia impacts their susceptibility to inactivation by ethanol vapours.

A common protocol employed for the preparation of conidia employs flooding a fungal colony grown on semi-solid media under optimum conditions with an aqueous solution. In contrast, conidia produced in a natural environment are usually not hydrated when disseminated in air and can be produced under water stress. In order to simulate the latter conditions, cultures were grown at different water activities and conidia were dry-harvested on the lid by turning the dishes upside-down then gently tapping the bottom of the box.

Inactivation of conidia of Penicillium chrysogenum, P. digitatum and P. italicum by ethanol solutions and vapours.

A fractional factorial design, 2(5-1) experiments, was used for assessing the influence of 5 factors: water activity, aw [0.7, 0.9], temperature, T ( degrees C) [10, 30], mode of application, A [liquid, vapour], ethanol concentration, E (% w/w) [5, 10] and time, t (d) [1, 4] on the inactivation of spores of Penicillium chrysogenum, P.