Impact of AbaI mutation on virulence, biofilm development, and antibiotic susceptibility in Acinetobacter baumannii.

The quorum sensing (QS) system mediated by the abaI gene in Acinetobacter baumannii is crucial for various physiological and pathogenic processes. In this study, we constructed a stable markerless abaI knockout mutant (ΔabaI) strain using a pEXKm5-based allele replacement method to investigate the impact of abaI on A. baumannii.

Investigation of antimicrobial resistance and antimicrobial resistance genes in and coagulase-negative staphylococci isolated from rabbit.

Background And Aim: Staphylococci, which inhabit skin and mucous membranes in humans and animals, are opportunistic pathogens. Coagulase-positive and coagulase-negative staphylococci (CoNS) are the two main groups. Clinical abscesses in rabbits often harbor and CoNS.

Unveiling the Antimicrobial, Anti-Biofilm, and Anti-Quorum-Sensing Potential of Linn. Leaf Extract against : An Integrated In Vitro-In Silico Investigation.

Antimicrobial resistance poses a global health threat, with emerging as a notorious pathogen capable of forming stubborn biofilms and regulating virulence through quorum sensing (QS). In the quest for novel therapeutic strategies, this groundbreaking study unveils the therapeutic potential of Linn., an Asian medicinal plant containing various bioactive compounds, contributing to its antimicrobial activities, in the battle against .

Type VI Secretion System Accessory Protein TagAB-5 Promotes Pathogenicity in Human Microglia.

Central nervous system (CNS) melioidosis caused by is being increasingly reported. Because of the high mortality associated with CNS melioidosis, understanding the underlying mechanism of pathogenesis in the CNS needs to be intensively investigated to develop better therapeutic strategies against this deadly disease. The type VI secretion system (T6SS) is a multiprotein machine that uses a spring-like mechanism to inject effectors into target cells to benefit the infection process.

Phenotypic and Genotypic Investigation of Carbapenem-Resistant in Maharaj Nakhon Si Thammarat Hospital, Thailand.

(1) Background: is well known as a causative agent of severe hospital-acquired infections, especially in intensive care units. The present study characterised the genetic traits of biofilm-forming carbapenem-resistant (CRAB) clinical isolates. Additionally, this study determined the prevalence of biofilm-producing isolates from a tertiary care hospital and investigated the association of biofilms with the distribution of biofilm-related and antibiotic resistance-associated genotypes.

Phylogenetic analysis and antibiotic resistance of isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand.

Background And Aim: Domestic and wild animals are important reservoirs for antibiotic-resistant bacteria. This study aimed to isolate from feces of domestic and wild animals at an agricultural land interface area of Salaphra Wildlife Sanctuary, Thailand, and study the phylogenic characteristics and antibiotic resistance in these isolates.

Materials And Methods: In this cross-sectional, descriptive study, we randomly collected ground feces from free-ranging wild animals (deer and elephants) and domestic animals (cattle and goats).

Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant isolated from healthy rabbits.

Background And Aim: Methicillin-resistant globally, (MRSA) is a major cause of disease in both humans and animals. Several studies have documented the presence of MRSA in healthy and infected animals. However, there is less information on MRSA occurrence in exotic pets, especially healthy rabbits.

Occurrence, antimicrobial resistance, virulence, and biofilm formation capacity of spp. and spp. isolated from raw seafood marketed in Bangkok, Thailand.

Background And Aim: Bacteria of the genera and cause seafood-borne zoonoses, which may have a significant impact on food safety, economy, and public health worldwide. The presence of drug-resistant and biofilm-forming phenotypes in the food chain increases the risk for consumers. This study aimed to investigate the characteristics, virulence, biofilm production, and dissemination of antimicrobial-resistant pathogens isolated from seafood markets in Bangkok, Thailand.

Development of a Rapid Reverse Transcription-Recombinase Polymerase Amplification Couple Nucleic Acid Lateral Flow Method for Detecting Porcine Epidemic Diarrhoea Virus.

Porcine epidemic diarrhea virus (PEDV) infection is an important acute diarrheal disease of swine that results in economic and industrial losses worldwide. The clinical manifestations in infected piglets are severe diarrhea, dehydration with milk curd indigestion, leading to death. The diagnosis of PEDV is essential for monitoring and managing the disease.

Antimicrobial Resistance and Virulence of Non-Typhoidal from Retail Foods Marketed in Bangkok, Thailand.

Nontyphoidal- bacteria cause foodborne gastroenteritis that may lead to fatal bacteremia, osteomyelitis, and meningitis if not treated properly. The emergence of multidrug-resistant strains is a global public health threat. Regular monitoring of genotypes and phenotypes of isolated from humans, animals, foods, and environments is mandatory for effective reduction and control of this food-borne pathogen.

Antibiotic Resistance Genes Among Carbapenem-resistant Enterobacterales (CRE) Isolates of Prapokklao Hospital, Chanthaburi Province, Thailand.

Background: The global spread of carbapenem-resistant Enterobacterales (CRE) inflicts a severe threat to human health. The CRE infections have resulted in an increased mortality rate in hospitals and other health-care settings worldwide. In this study, the antibiotic-resistance pattern and prevalence of carbapenemase-encoding genes among CRE isolated from patients of one hospital in Thailand were investigated.

Human Single-Chain Antibodies That Neutralize Elastolytic Activity of LasB.

LasB (elastase/pseudolysin) is an injurious zinc-metalloprotease secreted by the infecting . LasB is recognized as the bacterial key virulence factor for establishment of successful infection, acquisition of nutrients, dissemination, tissue invasion, and immune modulation and evasion. LasB digests a variety of the host tissue proteins, extracellular matrices, as well as components of both innate and adaptive immune systems, including immunoglobulins, complement proteins, and cytokines.

Screening method for identification in subclinical bovine mastitis from dairy farms.

Background: is one of the most important contagious bacteria causing subclinical bovine mastitis. This bacterial infection is commonly identified by determine the pathogen in bovine milk samples through conventional technique including coagulase test. However, this test has several disadvantages as low sensitivity, risk of biohazard, cost expensive, and limited preparation especially in local area.