Plant cell wall enzymatic deconstruction: Bridging the gap between micro and nano scales.

Understanding lignocellulosic biomass resistance to enzymatic deconstruction is crucial for its sustainable conversion into bioproducts. Despite scientific advances, quantitative morphological analysis of plant deconstruction at cell and tissue scales remains under-explored. In this study, an original pipeline is devised, involving four-dimensional (space  + time) fluorescence confocal imaging, and a novel computational tool, to track and quantify deconstruction at cell and tissue scales.