Publications by authors named "Thiago M Sanches"
Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While genotyping microarrays and reference panels for imputation are available for human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient and cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing.
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- Delay discounting is the tendency to prefer smaller, immediate rewards over larger rewards that take longer to receive, and it is linked to substance use disorders and mental health issues.
- A study using Heterogeneous Stock rats identified significant genetic loci on chromosomes 14 and 20 associated with delay discounting behavior via a genome-wide association study (GWAS).
- The genes found include Slc35f1, which may influence behavior through its expression levels, and Adgrl3, suggesting new genetic factors involved in delay discounting worthy of further investigation.
Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While genotyping microarrays and reference panels for imputation are available for human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient and cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing.
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- Age-related hearing impairment (ARHL) is a widespread condition among the elderly, influenced by environmental and genetic factors, and studying it in outbred mice can enhance our understanding of its molecular mechanisms.
- The study used Carworth Farms White (CFW) mice to analyze hearing changes at different ages, measuring their auditory response and collecting genetic data from over 4 million SNPs to identify genes linked to ARHL.
- Key findings included the discovery of several genetic regions associated with ARHL and the identification of the Prkag2 gene as a potential target for further research into treatments and prevention methods.
Tracking and quantifying the abundance and location of cells in the developing brain is essential in neuroscience research, enabling a greater understanding of mechanisms underlying nervous system morphogenesis. Widely used experimental methods to quantify cells labeled with fluorescent markers, such as immunohistochemistry (IHC), hybridization, and expression of transgenes via stable lines or transient electroporations (IUEs), depend on accurate and consistent quantification of images. Current methods to quantify fluorescently-labeled cells rely on labor-intensive manual counting approaches, such as the Fiji plugin , which requires custom macros to enable higher-throughput analyses.
View Article and Find Full Text PDFEnvironmental DNA (eDNA) analysis has gained traction as a precise and cost-effective method for species and waterways management. To date, publications on eDNA protocol optimization have focused primarily on DNA yield. Therefore, it has not been possible to evaluate the cost and speed of specific components of the eDNA protocol, such as water filtration and DNA extraction method when designing or choosing an eDNA protocol.
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