Platelet-rich fibrin/biphasic calcium phosphate impairs osteoclast differentiation and promotes apoptosis by the intrinsic mitochondrial pathway in chronic periodontitis.

Background: The study explored the effect of platelet-rich fibrin/biphasic calcium phosphate (PRF/BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of CP patients.

Methods: Peripheral blood mononuclear cells (PBMCs) from 25 patients with chronic periodontitis (CP) and 25 healthy individuals were assayed for cluster of differentiation14 (CD14 ) expression and monocytes were induced to differentiate into osteoclasts for 21 days in-vitro in the presence of macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-kappa B ligand (RANKL). We assessed the number of osteoclasts by tartrate-acid resistant acid phosphatase (TRAP)-positivity.