[Methadone-induced central adrenal insufficiency: a case report].

Adrenal insufficiency secondary to opioid use remains inadequately acknowledged in medical literature. We present the case of a 33-year-old female patient diagnosed with central adrenal insufficiency (CAI), where methadone use was identified as the underlying cause after ruling out known etiologies. This article aims to enhance awareness among prescribing clinicians and medical professionals regarding the potential occurrence of AI in patients undergoing methadone treatment.

Deciphering metabolic crosstalk in context: lessons from inflammatory diseases.

Metabolism plays a crucial role in regulating the function of immune cells in both health and disease, with altered metabolism contributing to the pathogenesis of cancer and many inflammatory diseases. The local microenvironment has a profound impact on the metabolism of immune cells. Therefore, immunological and metabolic heterogeneity as well as the spatial organization of cells in tissues should be taken into account when studying immunometabolism.

TNFR2 Costimulation Differentially Impacts Regulatory and Conventional CD4 T-Cell Metabolism.

CD4 conventional T cells (Tconvs) mediate adaptive immune responses, whereas regulatory T cells (Tregs) suppress those responses to safeguard the body from autoimmunity and inflammatory diseases. The opposing activities of Tconvs and Tregs depend on the stage of the immune response and their environment, with an orchestrating role for cytokine- and costimulatory receptors. Nutrient availability also impacts T-cell functionality metabolic and biosynthetic processes that are largely unexplored.

Preclinical Immune Response and Safety Evaluation of the Protein Subunit Vaccine Nanocovax for COVID-19.

The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global health concern. The development of vaccines with high immunogenicity and safety is crucial for controlling the global COVID-19 pandemic and preventing further illness and fatalities. Here, we report the development of a SARS-CoV-2 vaccine candidate, Nanocovax, based on recombinant protein production of the extracellular (soluble) portion of the spike (S) protein of SARS-CoV-2.

Lasibidoupins A and B, two new compounds from the stems of V.S. Dang & Naiki.

From the stems, two new compounds, including one new 9,10-anthraquinone, lasibidoupin A (), and one new 6,7-benzocoumarin, lasibidoupin B (), together with one known compound, 11--methyldamnacanthol () were isolated using chromatographic method. Their structures were determined by extensive HRMS, and NMR assignments. Compound was reported for the first time from this species.

Cycloartane-type triterpenoids from the whole plants of .

One new cycloartane-type triterpenoid, named macrobidoupoic acid A (as an C-24 epimeric mixture, , ), together with three known ones (-), were clarified by different chromatography from the whole plants. Triterpenoids ( & ) were detected for the first time from the genus. Chemical structures of them were illuminated using HR-ESI-MS, and NMR (1 D & 2 D) assessments.

Ricicomin A, a new alkaloid from the leaves of Linn.

From the leaves of Linn., one new alkaloid, named ricicomin A () together with three known ones, ricinine (), -demethylricinine () and 4-[2-formyl-5-(methoxymethyl)-1H-pyrrol-1-yl]butanoic acid () were justified by repeated chromatographic methods. Their structures were determined by comprehensive IR, HR-ESI-MS and NMR analyses.

Regulation of mating type switching by the mating type genes and RME1 in Ogataea polymorpha.

Saccharomyces cerevisiae and its closely related yeasts undergo mating type switching by replacing DNA sequences at the active mating type locus (MAT) with one of two silent mating type cassettes. Recently, a novel mode of mating type switching was reported in methylotrophic yeast, including Ogataea polymorpha, which utilizes chromosomal recombination between inverted-repeat sequences flanking two MAT loci. The inversion is highly regulated and occurs only when two requirements are met: haploidy and nutritional starvation.