Cloning and enhancing production of a detergent- and organic-solvent-resistant nattokinase from Bacillus subtilis VTCC-DVN-12-01 by using an eight-protease-gene-deficient Bacillus subtilis WB800.

Background: Nattokinases/Subtilisins (EC 3.4.21.

Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid.

Background: The lipase subfamilies I.1 and I.2 show more than 33% homology in the amino acid sequences and most members share another common property that their genes are clustered with the secondary genes whose protein products are required for folding the lipase into an active conformation and secretion into the culture medium.