Development and validation of a method for the analysis of hydroxyzine hydrochloride in extracellular solution used in in vitro preclinical safety studies.

In the process of drug development, preclinical safety studies are to be performed that require the analysis of the compound at very low concentrations with high demands on the performance of the analytical methods. In the current study, a UPLC-MS/MS method was developed and validated to quantify hydroxyzine hydrochloride in an extracellular solution used in a hERG assay in concentrations ranging from 0.01 to 10μM (4.

ICP-MS in drug development: high-throughput analysis of strontium and calcium in bone, tissue and plasma.

Background: The high-throughput analysis of strontium and calcium in plasma, tissue and bone (femur) using inductively coupled plasma-MS is described. Method validation, the results and experience obtained during sample analysis are highlighted.

Results: The different matrices were destructed with concentrated nitric acid by heating at 60°C overnight.