Repetitive ultramicrotome trimming and SEM imaging for characterizing printed multilayer structures.

Ultramicrotomy is a well-established technique that has been applied in biology and medical research to produce thin sections or a blockface of an embedded sample for microscopy. Recently, this technique has also been applied in materials science or micro- and nanotechnology as a sample preparation method for subsequent characterization. In this work, an application of ultramicrotomy for the cross-section preparation of an inkjet-printed multilayer structure is demonstrated.

Systematic Investigation of Novel, Controlled Low-Temperature Sintering Processes for Inkjet Printed Silver Nanoparticle Ink.

Functional inks enable manufacturing of flexible electronic devices by means of printing technology. Silver nanoparticle (Ag NP) ink is widely used for printing conductive components. A sintering process is required to obtain sufficient conductivity.

Experimental investigation on the change of pull-off force between bulk particulate material and an elastic polymeric filter fiber.

Hypothesis: Adhesion between particles and a filter fiber is an important process of the filtration as it dictates the process of separation and in the following the detachment process of particles during filter regeneration. In addition to the shear stress that a new polymeric stretchable filter fiber implements into the particulate structure, the elongation of the substrate (fiber) is also expected to cause a structural change in the surface of the polymer. Thus, the changed contact area and surface energy could affect the adhesion force between particles and fibers.

A comparison of simulation and traditional local anesthesia teaching methods among dental hygiene students.

Background: The COVID-19 pandemic forced dental educators to quickly modify the teaching-learning platform without testing outcomes of alternative teaching methods prior to implementation. One critical course affected was the teaching of local anesthesia (LA) that moved from practicing injections using the traditional student-to-student method to the simulation model using manikins.

Purpose: This study compared two LA teaching methods (student-to-student versus simulation) in two consecutive cohorts before and during the pandemic to assess differences in students' skill level and self-confidence.

Refined high-pressure tube design for improved resolution in high-pressure NMR spectroscopy.

High-pressure NMR spectroscopy has become a valuable analytical tool in many activities, including the energy sector, chemical research, and biophysics. Many pressure-based applications could benefit from low-field NMR. In this context, a novel, simple, and low-cost high-pressure tube design improving the signal-to-noise ratio by more than 80% is presented.

Influence of the Available Surface Area and Cell Elasticity on Bacterial Adhesion Forces on Highly Ordered Silicon Nanopillars.

Initial bacterial adhesion to solid surfaces is influenced by a multitude of different factors, e.g., roughness and stiffness, topography on the micro- and nanolevel, as well as chemical composition and wettability.

Enhancement of High-Resolution 3D Inkjet-Printing of Optical Freeform Surfaces Using Digital Twins.

3D-inkjet-printing is just beginning to take off in the optical field. Advantages of this technique include its fast and cost-efficient fabrication without tooling costs. However, there are still obstacles preventing 3D inkjet-printing from a broad usage in optics, e.

Artificial Intelligence Aided Design of Microtextured Surfaces: Application to Controlling Wettability.

Artificial intelligence (AI) has emerged as a powerful set of tools for engineering innovative materials. However, the AI-aided design of materials textures has not yet been researched in depth. In order to explore the potentials of AI for discovering innovative biointerfaces and engineering materials surfaces, especially for biomedical applications, this study focuses on the control of wettability through design-controlled hierarchical surfaces, whose design is supported and its performance predicted thanks to adequately structured and trained artificial neural networks (ANN).

Miniaturized high-throughput synthesis and screening of responsive hydrogels using nanoliter compartments.

The traditional pipeline of hydrogel development includes individual one-by-one synthesis and characterization of hydrogels. This approach is associated with the disadvantages of low-throughput and high cost. As an alternative approach to classical one-by-one synthesis, high-throughput development of hydrogels is still tremendously under-represented in the field of responsive material development, despite the urgent requirement for such techniques.

Assessment of high-resolution 3D printed optics for the use case of rotation optics.

We present design and manufacture of a 3D printed varifocal freeform optics. The optical refraction power can be tuned continuously by mutual rotation of two helically shaped lens bodies of azimuthally varying curvatures. Since no additional space for axial or lateral lens movement is required, rotation optics allow for a highly compact design of varifocal optics.

Initial Bacterial Adhesion Properties of Anodically Oxidized TiAlV.

This paper reports about the initial interaction of bacteria with anodically oxidized Ti6Al4V for the use as dental implant abutment surfaces. Ti6Al4V samples are anodically oxidized in hydrofluoric acid using different voltages. The resulting nanotopographies are characterized by atomic force microscopy, scanning electron microscopy and contact angle measurements.

Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions.

Peptide microarrays are a fast-developing field enabling the mapping of linear epitopes in the immune response to vaccinations or diseases and high throughput studying of protein-protein interactions. In this respect, a rapid label-free measurement of protein layer topographies in the array format is of great interest but is also a great challenge due to the extremely low aspect ratios of the peptide spots. We have demonstrated the potential of vertical scanning interferometry (VSI) for a detailed morphological analysis of peptide arrays and binding antibodies.

Study of Biofilm Growth on Slippery Liquid-Infused Porous Surfaces Made from Fluoropor.

Undesired growth of biofilms represents a fundamental problem for all surfaces in long-term contact with aqueous media. Mature biofilms resist most biocide treatments and often are a pathogenic threat. One way to prevent biofilm growth on surfaces is by using slippery liquid-infused porous surfaces (SLIPS).

Cannabidiol's Upregulation of -acyl Ethanolamines in the Central Nervous System Requires -acyl Phosphatidyl Ethanolamine-Specific Phospholipase D.

Δ-tetrahydrocannabinol (THC) and cannabidiol (CBD) are bioactive cannabinoids. We recently showed that acute THC administration drives region-dependent changes in the mouse brain lipidome. This study tested the hypothesis that cell lines representing cell types present in the central nervous system (CNS), neurons (N18 cells), astrocytes (C6 glioma cells), and microglia (BV2 cells) would respond differently to THC, CBD, or their combination.

Characterization of the microscopic tribological properties of sandfish () scales by atomic force microscopy.

Lizards of the genus are widely known under the common name sandfish due to their ability to swim in loose, aeolian sand. Some studies report that this fascinating property of sandfish is accompanied by unique tribological properties of their skin such as ultra-low adhesion, friction and wear. The majority of these reports, however, is based on experiments conducted with a non-standard granular tribometer.

Shear-Induced Detachment of Polystyrene Beads from SAM-Coated Surfaces.

In this work we experimentally and theoretically analyze the detachment of microscopic polystyrene beads from different self-assembled monolayer (SAM) surfaces in a shear flow in order to develop a mechanistic model for the removal of cells from surfaces. The detachment of the beads from the surface is treated as a thermally activated process applying an Arrhenius Ansatz to determine the activation barrier and attempt frequency of the rate determing step in bead removal. The statistical analysis of the experimental shear detachment data obtained in phosphate-buffered saline buffer results in an activation energy around 20 kJ/mol, which is orders of magnitude lower than the adhesion energy measured by atomic force microscopy (AFM).

A delta pH clamp method for analysis of steady-state kinetics of photophosphorylation.

An instrumental device is described which allows steady-state kinetic measurements of photophosphorylation at a desired proton gradient which can be maintained throughout the course of the experiment ('delta pH clamp'). This is achieved by electronic regulation of light intensity using the calibrated 9-aminoacridine fluorescence signal as sensor of the gradient. The instrument is suitable for determination of kinetic parameters of the proton-translocating ATPase in isolated envelope-free chloroplasts under defined conditions.

[Pseudarthrosis of the scaphoid: frequency, pathogenesis and course].

Eight-three scaphoid pseudo-arthroses were found amongst 1.104 scaphoid examinations. Sixty-seven were present at the first examination and 16 pseudoarthroses developed amongst 252 scaphoid fractures.