Publications by authors named "Thejaswi Bikkani"
Article Synopsis
- Up to one-third of patients with blood cancers who have received multiple transfusions develop immune-mediated issues that make platelet transfusions less effective, known as platelet transfusion refractoriness.
- This study analyzed 2012 platelet transfusions in 73 patients to examine how HLA antibodies and other patient factors influence the effectiveness of these transfusions, specifically looking at their impact on the corrected count increment (CCI) after 2 and 24 hours.
- Results showed that high levels of donor-specific antibodies, certain blood type mismatches, and other specific conditions negatively affected immediate posttransfusion platelet counts, suggesting that using a computerized algorithm for donor-recipient matching could improve outcomes in these patients.
Cell separation technologies play a vital role in the graft engineering of hematopoietic cellular fractions, particularly with the rapid expansion of the field of cellular therapeutics. The CliniMACS Plus Instrument (Miltenyi Biotec) utilizes immunomagnetic techniques to isolate hematopoietic progenitor cells (HPCs), T cells, NK cells, and monocytes. These products are ultimately used for HPC transplantation and for the manufacture of adoptive immunotherapies.
View Article and Find Full Text PDFBackground: When manufacturing chimeric antigen receptor (CAR) T cells using anti-CD3/anti-CD28 beads, ex vivo T-cell expansion is dependent on the composition of leukocytes used in the manufacturing process. We investigated the effects of leukocyte composition on CAR T-cell expansion and characteristics using an alternative manufacturing method.
Methods: Anti-B-cell maturation antigen and CD19-CAR T cells were manufactured using autologous peripheral blood mononuclear cell (PBMNC) concentrates.
Product safety assurance is crucial for the clinical use of manufactured cellular therapies. A rational approach for delivering products that fail release criteria (because of potentially false-positive sterility results) is important to avoid unwarranted wastage of highly personalized and costly therapies in critically ill patients where benefits may outweigh risk. Accurate and timely interpretation of microbial sterility assays represents a major challenge in cell therapies.
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