Optimization and characterization of immobilized thermostable α-amylase from germinating Sword bean ( (Jacq.) DC.) seeds on DEAE-cellulose and chitosan bead for operational stability.

Thermostable α-amylase from germinating Sword bean ( (Jacq.) DC.) seeds has been successfully immobilized on DEAE-cellulose (ICgAmy1) and chitosan bead (ICgAmy2) support materials.

Purification and characterization of thermostable α-amylase from germinating Sword bean ( (Jacq.) DC.) seeds.

The thermostable α-amylase from germinating sword bean ( (Jacq.) DC.) seeds (CgAmy) was successfully purified by a combination of ammonium sulphate fractionation and Epoxy-activated Sepharose 6B affinity chromatography.