Automated cell tracking using 3D nnUnet and Light Sheet Microscopy to quantify regional deformation in zebrafish.

Light Sheet Microscopy (LSM) in conjunction with embryonic zebrafish, is rapidly advancing three-dimensional, characterization of myocardial contractility. Preclinical cardiac deformation imaging is predominantly restricted to a low-order dimensionality image space (2D) or suffers from poor reproducibility. In this regard, LSM has enabled high throughput, non-invasive 4D (3d+time) characterization of dynamic organogenesis within the transparent zebrafish model.