Publications by authors named "Thawatchai Kitti"

Background: (. ) is a common pathogen of community-acquired pneumonia. Interleukin-17 (IL-17) plays a role in host defense and contributes to disease severity in infection.

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  • - This study used bioinformatics and phenotypic tests to analyze the features of a carbapenem-resistant bacteria strain, AB073, which showed resistance to seven different classes of antibiotics due to various mechanisms like altered targets and efflux.
  • - AB073 could not transfer its antibiotic resistance through conjugation, and genetic analysis found no links to horizontal gene transfer, indicating that its resistance traits are likely innate rather than acquired.
  • - The strain also possesses genes related to virulence factors, iron uptake, and biofilm formation, highlighting its successful spread as a pathogen in various regions and clinical settings, particularly originating from Thailand.
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  • * Research is focusing on new treatment methods using bacteriophages, specifically a newly isolated phage called vB_AbaAut_ChT04, which can infect many clinical strains of MDR-AB.
  • * Genetic analysis of vChT04 revealed a robust genome with antimicrobial potential, leading to the development of a peptide (PLysChT04) that demonstrated effectiveness against both MDR-AB and XDR-AB in laboratory tests.*
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  • The study investigates the genetic characteristics of a nosocomial pathogen commonly found in ICUs by comparing hospital environmental samples with clinical isolates to understand its resistance patterns.
  • Researchers collected 106 isolates from various hospital surfaces, finding high levels of multidrug resistance, particularly in AMBU bags, bedrails, and suction tubes, with environmental strains showing a notable degree of genetic similarity to clinical strains.
  • The findings highlight a significant presence of antibiotic resistance genes, with environmental and clinical strains frequently sharing genetic traits, raising concerns about infection control and transmissibility in hospital settings.
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Background: The global emergence and spread of extended-spectrum beta-lactamase (ESBL)-producing , especially and , have been recognized as a public health concern as severe infections caused by these microorganisms increase morbidity and mortality. This study aimed to assess the prevalence of ESBL-positive and strains isolated from hospitalized patients in Chiangrai Prachanukroh hospital, Chiangrai province, Thailand.

Methods: This retrospective analysis was conducted from January 2016 to December 2020.

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Escherichia coli, a bacterium that causes severe foodborne diseases, is transmitted to humans primarily through the consumption of contaminated foods. These foodborne pathogens are causing a public health problem that requires alternative control approaches, such as bacteriophage (phage) biocontrol. In this study, we characterized vB_EcoM_Tw01 (vTw01) isolated from sewage and vB_EcoM_Tcm05 (vTcm05) isolated from chicken meat.

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  • This study explored the link between antimicrobial resistance, CRISPR/Cas systems, and virulence related to phage susceptibility in Acinetobacter baumannii strains from Thailand.
  • Out of 230 strains tested, 46.5% were susceptible to phages, and this susceptibility was correlated with antibiotic resistance and biofilm formation, particularly linked to the ompA gene.
  • Whole-genome analysis showed that all phage-susceptible strains carried multiple antibiotic resistance genes, biofilm-related genes, and certain CRISPR-associated genes, highlighting the relationship between bacterial virulence and their evolutionary dynamics with phages.
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Extensively drug-resistant (XDR-AB) is a major threat to public health worldwide. A retrospective study for 27 XDR-AB isolates from four tertiary hospitals in Thailand was conducted. Beta-lactamase and virulence genes were characterized by PCR.

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Background: Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are multidrug-resistant bacteria that are difficult to treat because of their ability to form biofilms.

Objectives: In the present study, we evaluated the antibiotic-resistant phenotypes, biofilm-forming ability, and biofilm associated genes of 55 clinical MR-CoNS isolates obtained from two hospitals in Thailand.

Materials And Methods: MALDI-TOF-MS and gene sequencing were performed to determine the species of all isolates.

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Methicillin-resistant staphylococci are now recognized as a major cause of infectious diseases, particularly in hospitals. Molecular epidemiology is important for prevention and control of infection, but little information is available regarding staphylococcal infections in Northern Thailand. In the present study, we examined antimicrobial susceptibility patterns, detection of antimicrobial resistance genes, and SCC types of methicillin-resistant (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from patients in a hospital in Northern Thailand.

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Methicillin-resistant coagulase negative staphylococci (MR-CoNS) are the major cause of infectious diseases because of their potential ability to form biofilm and colonize the community or hospital environments. This study was designed to investigate the biofilm producing ability, and the presence of mecA, icaAD, bap and fnbA genes in MR-CoNS isolates. The MR-CoNS used in this study were isolated from various samples of community environment and five wards of hospital environments, using mannitol salt agar (MSA) supplemented with 4 μg/ml of oxacillin.

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Acinetobacter baumannii is a major threat to public health due to the emergence and dissemination of antibiotic-resistant strains. The purpose of this study was to determine the molecular epidemiology of antibiotic-resistant A. baumannii isolates collected from four tertiary hospitals in Thailand during the period November 2013-February 2015.

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Endolysins are lytic enzymes produced by bacteriophages with their ability to degrade the cell wall of bacterial hosts. Endolysin (LysABP-01) from Acinetobacter baumannii bacteriophage ØABP-01 was cloned, overexpressed and characterized. Endolysin LysABP-01 has a globular structure consisting of lysozyme-like (N-acetyl-β-D-muramidase) catalytic domain.

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Acinetobacter baumannii is an opportunistic pathogen that exists in hospital environments. The emergence of multidrug resistant A. baumannii (MDRAB) has been reported worldwide.

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Staphylococcus aureus is a bacterium causing infections in both community and hospitals. S. aureus nasal colonization increases the risk of acquiring S.

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We studied the prevalence of methicillin sensitive Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA) nasal colonization among healthy young Thai adults. MSSA nasal colonization was found in 30 of 200 subjects (15%). The prevalence of MRSAnasal carriage was 1% (2 of 200) detected by cefoxitin/oxacillin disk diffusion and oxacillin salt screening methods.

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McsA is a key modulator of stress response in Staphylococcus aureus that contains four CXXC potential metal-binding motifs at the N-terminal. Staphylococcus aureus ctsR operon encodes ctsR, clpC, and putative mcsA and mcsB genes. The expression of the ctsR operon in S.

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