Evidence-Triggers for Care of Patient with Cleft Lip and Palate at Srinagarind Hospital: Antenatal Care Unit.

Background: The abnormalities of cleft lip and palate (CLP) condition are the serious problems which always found in the northeastern region of Thailand. The treatment must be sustainably and continuously integrated by the interdisciplinary team, including registered nurses who take care of all nine organizations under the corporation among nurses who work at Tawanchai Cleft Center. Starting from the diagnosis of the fetus by the new technology along with medical knowledge which can diagnose the abnormalities since birth.

Second trimester genetic ultrasound for Down syndrome screening at Srinagarind Hospital.

Objective: To assess the value of second trimester genetic ultrasound for screening of Down syndrome conducted at Srinagarind Hospital, Khon Kaen, Thailand.

Material And Method: The present study sample comprised of 4,033 pregnant women at high risk forfetal chromosomal abnormality, from 17th to 23th week, who had performed second trimester genetic ultrasound before genetic amniocentesis between September 1996 and December 2011. Archived medical records relating to results ofgenetic ultrasound and genetic amniocentesis were extracted and studied.

Effect of knowledge and perception on adherence to iron and folate supplementation during pregnancy in Kathmandu, Nepal.

Background: Adherence to iron and folate supplementation during pregnancy is considered key to prevention and control of iron deficiency anemia. Nepal-like other developing countries-faces problems with adherence vis-a-vis iron/folate supplementation.

Objective: This descriptive survey aimed to assess the effect ofknowledge and perception ofpregnant women on adherence to iron/folate supplementation in Kathmandu, Nepal.

Fetal red blood cell parameters in thalassemia and hemoglobinopathies.

Introduction: With the lack of fetal blood specimens in routine practice, little is known about red blood cell (RBC) parameters of fetuses with various thalassemia syndromes. This study aimed to describe these in various forms of thalassemia.

Materials And Methods: The study was performed on 93 fetal blood specimens obtained from pregnant women by cordocentesis during 18-24 weeks of gestation.

Prenatal three dimensional ultrasonography and expectant management of placental chorioangioma: a case report.

A 24-year-old Thai woman presented with large for date. Two dimensional (2D) and Doppler ultrasonography revealed a large placental mass with prominent vasculature suggestive of chorioangioma with polyhydramnios. Three-dimensional (3D) ultrasonography was used to demonstrate the better images for parental counseling.

Effectiveness of the model for prenatal control of severe thalassemia.

Objective: The aim of the research was to determine effectiveness of the model for prenatal control in reducing new cases of severe thalassemia.

Methods: Pregnant women at six tertiary centers were recruited to follow the model, consisting of (1) carrier screening using mean corpuscular volume (for alpha-thal-1 and beta-thal) and CMU-E screen (for HbE trait), (2) carrier diagnosis, (3) the couples at risk were counseled and offered prenatal diagnosis, and (4) termination of affected pregnancy. All neonates were evaluated for thalassemia.

Normogram of fetal middle cerebral artery peak systolic velocity at 20-37 weeks of gestation at Srinagarind Hospital.

Objective: To establish normal references of fetal middle cerebral artery peak systolic velocity (MCA PSV) at 20 to 37 weeks of gestation at Srinagarind Hospital.

Material And Method: A descriptive cross-sectional study was performed Normal fetuses at 20 to 37 weeks of gestation were studied by gray scale ultrasonography initially and then Doppler of MCA PSV.

Results: At least 18 pregnant women of each gestational age with an uncomplicated singleton were enrolled The MCA PSV was increased as gestational age advanced from 24.

Fetal pleural effusions with spontaneous resolution: a case report.

A 42-year-old pregnant woman was referred with massive fetal bilateral pleural effusions. Observation with serial ultrasound was made. The documented spontaneous resolution of fetal pleural effusion was recorded.

Distribution of normal nuchal translucency thickness: a multicenter study in Thailand.

Aims: To establish the normative data distribution of nuchal translucency (NT) thickness in Thai fetuses.

Methods: A cross-sectional multicenter study was conducted among 6,455 women with singleton pregnancies and gestational age between 10 and 14 weeks. For each case, the fetal crown-rump length (CRL) and NT were measured by transabdominal ultrasound.

Prevention of severe thalassemia in northeast Thailand: 16 years of experience at a single university center.

Objective: To demonstrate the performance of thalassemia prevention in northeast Thailand during 1993-2008.

Methods: Retrospective data from 1422 at-risk couples who attended from January 1993 to December 2008 were studied. All couples were suspected at-risk couples based on initial screening using standard protocols.

Accurate prenatal diagnosis of Hb Bart's hydrops fetalis in daily practice with a double-check PCR system.

Hemoglobin (Hb) Bart's hydrops fetalis is a fatal condition associated with homozygous alpha(0)-thalassemia. Prenatal diagnosis of the disease is usually done by gap-PCR; however, misdiagnosis can occur with allelic dropout. Diagnosis using more than one method is preferred.

Incidence, causes and pregnancy outcomes of hydrops fetalis at Srinagarind Hospital, 1996-2005: a 10-year review.

Objective: To identify the incidence and determine causes and pregnancy outcomes of hydrops fetalis at Srinagarind Hospital.

Study Design: A retrospective descriptive study.

Setting: Department of Obstetrics and Gynecology, Faculty of Medicine, Srinagarind Hospital, Khon Kaen University.

Analysis of fetal blood using capillary electrophoresis system: a simple method for prenatal diagnosis of severe thalassemia diseases.

Introduction: Prenatal diagnosis of severe alpha- and beta-thalasssemia diseases is usually performed by DNA analysis.

Objective: To establish a simple method, we have evaluated the reliability of prenatal diagnosis by fetal blood analysis using automated capillary electrophoresis system.

Methods: Forty-seven fetal blood specimens collected by cordocentesis at 18-28 wk of gestation were analyzed by the capillary electrophoresis system (Sebia).

Accuracy of fetal gender detection using a conventional nested PCR assay of maternal plasma in daily practice.

We have prospectively examined the diagnostic accuracy of a conventional multiplex polymerase chain reaction (PCR) analysis of maternal plasma for fetal gender determination in daily practice. Plasma DNAs were obtained from 168 pregnant women between five and 32 weeks of gestation. The 198-bp-specific sequence on Y-chromosome and the 261 bp ATL1-gene-specific sequence on X-chromosome were coamplified in a multiplex nested PCR manner.

Fetal biometry charts for normal pregnant women in northeastern Thailand.

Objectives: 1) To construct normal fetal biometry charts of fetal biparietal diameter (BPD), head circumference (HC), abdominal circumference (AC), and femur length (FL) from 14 to 41 weeks of gestation in northeastern Thailand. 2) To compare the results with other studies.

Study Design: A cross sectional descriptive study.

Application of maternal plasma DNA analysis for noninvasive prenatal diagnosis of Hb E-beta-thalassemia.

To establish simple noninvasive prenatal diagnosis of common beta-thalassemia in Southeast Asia, we have evaluated the possibility of identifying the 3 most common beta-thalassemia genes [beta(E), beta(17A-T), and beta(41/42(-CTCC))] by analysis of fetal DNA in maternal plasma using combined conventional polymerase chain reaction (PCR) and real-time quantitative PCR. Maternal plasma was obtained from peripheral blood of Thai pregnant women collected during the first and second trimesters of gestation. DNA was prepared from 200 microL plasma using a QIAmp Blood Mini Kit.

Prenatal prevention for severe thalassemia disease at Srinagarind Hospital.

Objective: To evaluate the results and cost-effectiveness of prenatal prevention measurement in severe thalassemia diseases at Srinagarind Hospital.

Study Design: Descriptive study.

Setting: Antenatal care (ANC) Clinic, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University.

Autopsy findings of fetal death.

Objective: To study the autopsy findings associated with fetal death in the division of reproductive pathology.

Material And Method: Descriptive study of 35 fetal deaths with placentas after postmortem examinations in the division of reproductive pathology between January 2005 and December 2005. The fetal deaths and placentas were examined by a perinatal pathologist in the surgical pathology room, Department of Pathology and Department of Obstetrics and Gynecology, Faculty of Medicine, Khon Kaen University.

Effect of the maternal betaE-globin gene on hematologic responses to iron supplementation during pregnancy.

Background: It is customary in Southeast Asia to treat pregnant anemic women with iron supplements, but anemia in this region may be complicated by thalassemia and hemoglobinopathies, which lead to an ineffective response.

Objective: The aim was to determine whether routine iron supplementation during pregnancy in this area, which has a high prevalence of thalassemia and hemoglobinopathies, is an effective control strategy for iron deficiency anemia.

Design: A prospective study was conducted.

Lived experiences of mothers caring for children with thalassemia major in Thailand.

Issues And Purpose: Thalassemic patients must be given continuous treatment throughout their lives due to the physical and psychological effects of their disorder; their families also are impacted. This qualitative study explored the lived experiences of 15 mothers of children with thalassemia major by conducting semistructured interviews; the data were analyzed utilizing content analysis.

Conclusion: Six themes were identified: lack of knowledge about thalassemia, psychosocial problems, concerns for the future, social support systems, financial difficulty, and the effectiveness of healthcare services.

Development and application of a real-time quantitative PCR for prenatal detection of fetal alpha(0)-thalassemia from maternal plasma.

In order to provide a noninvasive prenatal diagnosis of alpha(0)-Thalassemia (Southeast Asian [SEA] deletion), we have developed a real-time quantitative semi-nested polymerase chain reaction (PCR) method for identifying the fetal alpha(0)-Thalassemia in maternal plasma. Analysis was performed using DNA extracted from 200 muL plasma from 13 pregnant women during 8-20 weeks of gestation who carried fetuses with normal (2), alpha(0)-Thalassemia carrier (8), Hb H disease (1), and homozygous alpha(0)-Thalassemia (Hb Bart's hydrops fetalis (2). The alpha(0)-Thalassemia was detected using a two-step PCR.

Hydrops fetalis caused by parvovirus B19 infection: case report and literature review.

An intrauterine parvovirus B19 infection can result in severe fetal anemia and hydrops fetalis, which can lead to death. A case of fetal hydrops, diagnosed at 31 weeks gestation, is reported Cordocentesis revealed fetal hemoglobin of 5 g/dL. Due to fetal distress 18 hours later, the baby was delivered by emergency cesarean section and died two days later.

Thalassemia and hemoglobinopathies rather than iron deficiency are major causes of pregnancy-related anemia in northeast Thailand.

A cross-sectional prevalence study of anemia was undertaken on 412 pregnant women in northeast Thailand during January 2003 to May 2004. With standardized diagnostic protocols and the CDC criteria of anemia [hemoglobin (Hb) < 11 g/dl at < or = 12 weeks of gestation and Hb < 10.5 g/dl at < or = 20 weeks of gestation], 71 (17.

Correlation of weight estimation in large and small fetuses with three-dimensional ultrasonographic volume measurements of the fetal upper-arm and thigh: A preliminary report.

Objective: To determine the correlation of measuring fetal upper-arm and thigh volume using three-dimensional ultrasonography with weight estimation in large and small fetuses.

Material And Method: The authors included 46 pregnant women admitted for delivery in the Labor Room at Srinagarind Hospital, Khon Kaen University, between February 1 and September 30, 2004. Inclusion criteria were: 1) singleton pregnancy; 2) delivery within 48 hours after study; 3) estimation birthweight < 2,500 g (small fetuses group: n = 22) or > 3,500 g (large fetus group: n = 24).

Variability of nuchal translucency measurement: a multicenter study in Thailand.

Objectives: To evaluate intra- and inter-observer variations of nuchal translucency (NT) measurements and study the duration of measurements.

Subjects: One hundred and forty-seven singleton pregnant women with 10-14 weeks of gestation who had attended antenatal clinic during January 1st, 2000-August 31st, 2001 were included.

Methods: Crown-rump length and NT were measured three times for each woman.