Publications by authors named "Thassanai Sitthiyotha"
Brought about by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), coronavirus disease (COVID-19) pandemic has resulted in large numbers of worldwide deaths and cases. Several SARS-CoV-2 variants have evolved, and Omicron (B.1.
View Article and Find Full Text PDFDuring the COVID-19 pandemic, SARS-CoV-2 has caused large numbers of morbidity and mortality, and the Omicron variant (B.1.1.
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- Intelectins are immune lectins found in chordates, with XCGL-1 being the first identified, crucial for fertilization membrane development.
- Researchers studied XCGL-1's biochemical properties, revealing its four-lobed structure and the significance of cysteines for disulfide bond formation.
- XCGL-1 binds galactose-containing carbohydrates with high affinity and the findings could lead to new applications in glycobiology.
α-L-rhamnosidase catalyzes hydrolysis of the terminal α-L-rhamnose from various natural rhamnoglycosides, including naringin and hesperidin, and has various applications such as debittering of citrus juices in the food industry and flavonoid derhamnosylation in the pharmaceutical industry. However, its activity is lost at high temperatures, limiting its usage. To improve Lactobacillus acidophilus α-L-rhamnosidase stability, we employed molecular dynamics (MD) to identify a highly flexible region, as evaluated by its root mean square fluctuation (RMSF) value, and computational protein design (Rosetta) to increase rigidity and favorable interactions of residues in highly flexible regions.
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- Intelectins are immune proteins found in chordates, including fish, that are activated during infections; however, their specific functions and properties in fish are not fully understood.
- The study focuses on zebrafish intelectin-2 (DrIntL-2), which is mainly found in the gastrointestinal tract, revealing its structure and binding characteristics with bacteria.
- Findings suggest DrIntL-2 could play a vital role in fish immune response and has potential applications in detecting and identifying harmful bacteria, benefiting the aquaculture industry.
SARS-CoV-2 is responsible for COVID-19 pandemic, causing large numbers of cases and deaths. It initiates entry into human cells by binding to the peptidase domain of angiotensin-converting enzyme 2 (ACE2) receptor via its receptor binding domain of S1 subunit of spike protein (SARS-CoV-2-RBD). Employing neutralizing antibodies to prevent binding between SARS-CoV-2-RBD and ACE2 is an effective COVID-19 therapeutic solution.
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- Pfs25 is a key candidate for malaria transmission-blocking vaccines, but its genetic diversity has not been thoroughly studied in various endemic populations.
- A comprehensive analysis of 307 Pfs25 sequences revealed 11 unique haplotypes, with two dominant ones (H1 and H2) making up the majority and showing region-specific prevalence.
- The study identified specific single-nucleotide polymorphisms (SNPs) contributing to this diversity, with implications for the effectiveness of vaccines across different geographical regions.
SARS-CoV-2 is coronavirus causing COVID-19 pandemic. To enter human cells, receptor binding domain of S1 subunit of SARS-CoV-2 (SARS-CoV-2-RBD) binds to peptidase domain (PD) of angiotensin-converting enzyme 2 (ACE2) receptor. Employing peptides to inhibit binding between SARS-CoV-2-RBD and ACE2-PD is a therapeutic solution for COVID-19.
View Article and Find Full Text PDFSARS-CoV-2 is the novel coronavirus causing the COVID-19 pandemic. To enter human cells, the receptor-binding domain (RBD) of the S1 subunit of SARS-CoV-2 (SARS-CoV-2-RBD) initially binds to the peptidase domain of angiotensin-converting enzyme 2 receptor (ACE2-PD). Using peptides to inhibit SARS-CoV-2-RBD binding to ACE2 is a potential therapeutic solution for COVID-19.
View Article and Find Full Text PDFLipopolysaccharide (LPS) is a crucial component in the outer membrane of Gram-negative bacteria that contributes to both pathogenicity as well as immunity against pathogenic bacteria. Typical LPS contains GlcN disaccharide as the core of lipid A. However, some bacteria such as and contain GlcN3N in lipid A instead.
View Article and Find Full Text PDFLevansucrase (LS) from Gram-positive bacteria generally produces a large quantity of levan polymer, a polyfructose with glucose at the end (GF) but a small quantity of levan-type fructooligosaccharides (LFOs). The properties of levan and LFOs depend on their chain lengths, thereby determining their potential applications in food and pharmaceutical industries such as prebiotics and anti-tumor agents. Therefore, an ability to redesign and engineer the active site of levansucrase for synthesis of products with desired degree of polymerization (DP) is very beneficial.
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- Levan and its oligosaccharides, produced by levansucrase, show promise for use in the food and pharmaceutical industries for functions like prebiotics and anti-tumor agents.
- Mutations in Bacillus licheniformis RN-01 levansucrase (specifically N251A and N251Y) enable the production of short-chain oligosaccharides (up to GF3), but hinder the formation of long-chain levan due to decreased binding affinity for GF3.
- Molecular dynamics analysis reveals that these mutations do not significantly affect GF2 binding but significantly disrupt GF3 binding, highlighting the role of Arg255 in the levansucrase active site and offering insights into the mechanisms of oligosaccharide production