A Pipeline for the Development of Microsatellite Markers using Next Generation Sequencing Data.

Also known as Simple Sequence Repetitions (SSRs), microsatellites are profoundly informative molecular markers and powerful tools in genetics and ecology studies on plants. This research presents a workflow for developing microsatellite markers using genome skimming. The pipeline was proposed in several stages that must be performed sequentially: obtaining DNA sequences, identifying microsatellite regions, designing primers, and selecting candidate microsatellite regions to develop the markers.