Antibiotic potential and metabolic modulation of Bacillus velezensis VTRNT 01 in response to bacterial elicitors.

Bacterial elicitors are recognized for their ecological role in stimulating plant defenses and enhancing the production of beneficial metabolites. This study explores the antibiotic potential of endophytic Bacillus velezensis VTRNT 01, isolated from Adenosma bracteosum Bonati, under co-cultivation with bacterial elicitors (Staphylococcus aureus, Escherichia coli, and Aeromonas hydrophila). By leveraging these interactions, we aim to unlock the full potential of endophytic bacteria for sustainable applications in agriculture and pharmaceuticals.

Trans-Galen Approach for Embolization of Sphenoparietal Sinus Dural Arteriovenous Fistulas.

A patient in their early 60s presented with a headache and right-sided numbness. Imaging revealed a hemorrhagic lesion in the left thalamus and venous engorgement involving the left sphenoparietal sinus, with drainage through the basal vein of Rosenthal and the superficial middle cerebral vein. The trans-Galen approach was utilized for embolization, successfully obliterating the fistulas using coils without retrograde opacification.

Regulating ferredoxin electron transfer using nanobody and antigen interactions.

Fission and fusion can be used to generate new regulatory functions in proteins. This approach has been used to create ferredoxins (Fd) whose cellular electron transfer is dependent upon small molecule binding. To investigate whether Fd fragments can be used to monitor macromolecular binding reactions, we investigated the effects of fusing fragments of Fd to single domain antibodies, also known as nanobodies, and their protein antigens.

Red teaming ChatGPT in medicine to yield real-world insights on model behavior.

Red teaming, the practice of adversarially exposing unexpected or undesired model behaviors, is critical towards improving equity and accuracy of large language models, but non-model creator-affiliated red teaming is scant in healthcare. We convened teams of clinicians, medical and engineering students, and technical professionals (80 participants total) to stress-test models with real-world clinical cases and categorize inappropriate responses along axes of safety, privacy, hallucinations/accuracy, and bias. Six medically-trained reviewers re-analyzed prompt-response pairs and added qualitative annotations.

The outcomes of pediatric extracranial malignant germ cell tumors: A decade of experience from a single institution in Southern Vietnam.

Background: Extracranial malignant germ cell tumors (GCTs) generally exhibit favorable outcomes with contemporary therapeutic approaches. However, the outcomes of pediatric GCTs in Vietnam remain unclear. This study aims to evaluate the clinical features, treatment modalities, and prognostic factors associated with survival outcomes in children with GCTs treated at Children's Hospital Number 2 (CH2) in Ho Chi Minh City, Vietnam.

Genetic Landscape of Mucosal Melanoma: Identifying Pathogenic Germline Variants.

Mucosal melanomas (MM) are rare but aggressive malignancies, comprising only 1.3% of all melanoma diagnoses, with a poor 5-year survival rate below 20%. MM lacks identifiable risk factors, presents with distinct mutational profiles, and is often diagnosed at an advanced stage, contributing to worse outcomes.

Fabrication and evaluation of BerNPs regarding the growth and development of .

In this study, berberine nanoparticles (BerNPs) were prepared using a wet-milling method with zirconium balls to enhance bioavailability and expand potential applications. The particle size and physicochemical properties of the BerNPs were analyzed using field-emission scanning electron microscopy (FE-SEM), UV-vis spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The broth dilution method was used to determine the antimicrobial activity of the BerNPs against ().

Efficient In-Training Adaptive Compound Loss Function Contribution Control for Medical Image Segmentation.

Image segmentation plays a crucial role in many clinical applications, including disease diagnosis and monitoring. Current state-of-the-art segmentation approaches use deep neural networks that are trained on their target tasks by minimizing a loss function. Class imbalance is one of the major challenges that these networks face, where the target object is significantly underrepresented.

Computational analysis of light diffusion and thermal effects during Transcranial Photobiomodulation.

Transcranial Photobiomodulation (tPBM) is a non-invasive procedure where light is applied to the scalp to modulate underlying brain activity. tPBM has recently attracted immense interest as a potential therapeutic option for a range of neurological and neuropsychiatric conditions. The common technological questions related to this modality are extent of light penetration and associated scalp and brain temperature increases.

Photobleaching Step Counting and Localization for Fluorescence Microscopy.

Fluorescence microscopy is a fundamental tool for studying biological systems at the single-molecule level. In particular, photobleaching step patterns can provide valuable insights into molecule interactions. Several works have proposed an automated and robust way to estimate when photobleaching steps occur.

Randomised controlled trial of music listening combined with progressive muscle relaxation for mood management in women receiving chemotherapy for cancer.

Aims: To evaluate the effects of passive music listening combined with progressive muscle relaxation on anxiety, depression, stress, coping, and quality-of-life in women with breast and gynaecological cancers receiving chemotherapy.

Methods: This was an assessor-blinded, randomised wait-list controlled trial. A total of 120 participants were randomly allocated into an intervention group or a wait-list control group.

Short-Term Outcomes Using a Novel Femtosecond Laser-Assisted Keratotomy Nomogram to Manage Corneal Astigmatism During Phacoemulsification.

Purpose: To evaluate the efficacy of a femtosecond laser assisted astigmatic keratotomy (AK) nomogram (FemtoAK.com) for correction of astigmatism during cataract surgery.

Patients And Methods: Consecutive patients underwent cataract extraction with AKs and insertion of a non-toric intraocular lens.

Impact of Therapeutic Plasma Exchange and Rituximab for Prevention of Idiopathic Focal Segmental Glomerulosclerosis Recurrence Post-Kidney Transplantation.

Background: Focal segmental glomerulosclerosis (FSGS) recurs after kidney transplantation (KT) in 30%-50% of recipients. Recurrence is associated with early graft loss in up to 60% of cases. This study aimed to assess the efficacy of therapeutic plasma exchange (TPE) combined with rituximab (RTX) in preventing early FSGS recurrence within 1 y post-KT.

Predictive validity of the sequential organ failure assessment score for mortality in patients with acute respiratory distress syndrome in Vietnam.

Evaluating the prognosis of ARDS patients using grading systems can enhance treatment decisions. This retrospective observational study evaluated the predictive accuracy of the SOFA score, APACHE II score, SpO/FiO ratio, and PaO/FiO ratio for mortality in ARDS patients in Vietnam. The study included 335 adult ARDS patients admitted to a central hospital from August 2015 to August 2023.

Correction: Optical response of magnetically actuated biocompatible membranes.

Correction for 'Optical response of magnetically actuated biocompatible membranes' by H. Joisten , , 2019, , 10667-10683, https://doi.org/10.

Disruption of the moonlighting function of CTF18 in a patient with T-lymphopenia.

Introduction: Newborn screening for immunodeficiency has led to the identification of numerous cases for which the causal etiology is unknown.

Methods: Here we report the diagnosis of T lymphopenia of unknown etiology in a male proband. Whole exome sequencing (WES) was employed to nominate candidate variants, which were then analyzed functionally in zebrafish and in mice bearing orthologous mutations.

Search for Magnetic Monopole Pair Production in Ultraperipheral Pb+Pb Collisions at sqrt[s_{NN}]=5.36 TeV with the ATLAS Detector at the LHC.

This Letter presents a search for highly ionizing magnetic monopoles in 262  μb^{-1} of ultraperipheral Pb+Pb collision data at sqrt[s_{NN}]=5.36  TeV collected by the ATLAS detector at the LHC. A new methodology that exploits the properties of clusters of hits reconstructed in the innermost silicon detector layers is introduced to study highly ionizing particles in heavy-ion data.

MSConnect: Open-Source, End-to-End Platform for Automated Mass Spectrometry Data Management, Analysis, and Visualization.

The scale of mass spectrometry-based proteomics data sets continues to increase, and the analysis workflows are becoming more complex as various steps are carried out using a multitude of software programs developed by both commercial providers and the research community. Manually shepherding data across multiple programs and in-house-developed scripts can be error prone and labor intensive. It is also difficult for others to follow the same steps, leading to poor repeatability.

Embedded bioprinting of dense cellular constructs in bone allograft-enhanced hydrogel matrices for bone tissue engineering.

Bone tissue engineering aims to address critical-sized defects by developing biomimetic scaffolds that promote repair and regeneration. This study introduces a material extrusion-based embedded bioprinting approach to fabricate dense cellular constructs within methacrylated hyaluronic acid (MeHA) hydrogels enhanced with bioactive microparticles. Composite matrices containing human bone allograft or tricalcium phosphate (TCP) particles were evaluated for their rheological, mechanical, and osteoinductive properties.

The Use of Direct Current Cardioversion for Unstable Atrial Fibrillation with Rapid Ventricular Response in Critically ill Patients - a Propensity Score Analysis.

Objectives: There is substantial practice variation in the management strategies for atrial fibrillation (AF) with rapid ventricular rate (RVR) and hemodynamic instability in critically ill patients. This study aimed to evaluate the use and effectiveness of direct current cardioversion (DCCV) for unstable AF RVR in the intensive care unit (ICU).

Design: Multicenter retrospective cohort study.

A curated soil fungal dataset to advance fungal ecology and conservation research in Australia and Antarctica.

DNA metabarcoding has played a pivotal role in advancing our understanding of the diversity and function of soil-inhabiting fungi. The Australian Microbiome Initiative has produced an extensive soil fungal metabarcoding dataset of more than 2000 plots across a breadth of ecosystems in Australia and Antarctica. Sequence data requires rigorous approaches for the integration of species occurrences into biodiversity platforms, addressing biases due to false positives or overinflated diversity estimates, among others.

Optimizing Wound Culture Ordering in Electronic Health Records: A Case Report.

Background: At a large quaternary health system, tissue specimens were frequently sent to the microbiology laboratory with an incorrect wound culture order meant for swab specimens due to poor electronic health record menu design. Wound cultures were also requested in chronic wound cases with a low index of suspicion for acute infection.

Objective: To present a case report on specific changes to the design of the electronic test menu that resulted in higher numbers of appropriate ordering practices.