Three undescribed phenylbutenoids derivatives from Roxb. rhizomes and their biological activities.

Phenylbutenoids belong to polyphenolic compounds that have demonstrated distinctive biological activities and are primarily characterised in the genus Zingiber (Zingiberaceae) species. From the EtOAc extract of the rhizomes of Roxb., three phenylbutenoid-type compounds, cassudimin B (), cassumunol O (), and cassumunol P (), were isolated, along with five known compounds (-).

A novel diphenylbutenoid-type compound from the rhizomes of (J.Koenig) Link ex A.Dietr. (Zingiberaceae).

From the EtOAc-soluble extract of the rhizomes of (J.Koenig) Link ex A.Dietr.

Autophagy Inhibitor Chloroquine Downmodulates Hepatic Stellate Cell Activation and Liver Damage in Bile-Duct-Ligated Mice.

Hepatic stellate cell (HSC) activation via the autophagy pathway is a critical factor in liver fibrogenesis. This study tests the hypothesis that chloroquine (CQ) treatment can prevent autophagy and HSC activation in vitro and in vivo in bile-duct-ligated (BDL) mice. Sham-operated and BDL mice were treated with either PBS or CQ in two 60 mg/kg doses the day (D) before and after surgery.

Optimization of the isolation procedure and culturing conditions for hepatic stellate cells obtained from mouse.

Liver fibrosis (LF) mortality rate is approximately 2 million per year. Irrespective of the etiology of LF, a key element in its development is the transition of hepatic stellate cells (HSCs) from a quiescent phenotype to a myofibroblast-like cell with the production of fibrotic proteins. It is necessary to define optimal isolation and culturing conditions for good HSCs yield and proper phenotype preservation for studying the activation of HSCs in vitro.