Effect of cooking temperature and time on the quality and safety of hemp seed oil.

Hemp seed oil, known for its abundance in polyunsaturated fatty acids, is considered an emerging nutritious alternative to conventional edible oils available in the market. However, the knowledge about the oil quality and stability during cooking is limited. The impact of different cooking temperatures (170 °C, 190 °C, 210 °C, 230 °C) and times (15 min, 30 min, 1 h, 2 h, 3 h) on the fatty acid profiles and physicochemical properties (peroxide and acid values) of three types of hemp seed oil (original hemp oil, garlic infused hemp oil, chili infused hemp oil) were investigated in this study.

Discovery and characterization of new O-methyltransferase from the genome of the lignin-degrading fungus Phanerochaete chrysosporium for enhanced lignin degradation.

Using bioinformatic homology search tools, this study utilized sequence phylogeny, gene organization and conserved motifs to identify members of the family of O-methyltransferases from lignin-degrading fungus Phanerochaete chrysosporium. The heterologous expression and characterization of O-methyltransferases from P. chrysosporium were studied.

Inactivating effect of phenolic unit structures on the biodegradation of lignin by lignin peroxidase from Phanerochaete chrysosporium.

An imbalance of electron in an intramolecular electron transfer pathway was identified as the central factor causing inefficient degradation of lignin by the lignin peroxidase H8 from Phanerochaete chrysosporium (LiPH8). It was elucidated that dimeric lignins or monolignolic analogs containing free-hydroxyl phenolic groups were not only favorable substrates for the reduction of LiPH8 but also strong inhibitors depressing the enzymatic degradation of lignin. The data collectively demonstrated that disturbing the interaction between the free OH group on the phenolic structure and the surface active sites around Trp171 caused the primary deficiency in electron transport between Trp171 and the heme site, which severely inhibited the efficiency of lignin biodegradation by LiPH8/H2O2.