Evaluation and Optimization of physical, mechanical, and biological characteristics of 3D printed Whitlockite/Calcium Silicate composite scaffold for bone tissue regeneration using Response Surface Methodology.

Calcium phosphate (CaP)-based bioscaffolds are used for bone tissue regeneration because of their physical and chemical resemblance to human bone. Calcium, phosphate, sodium, potassium, magnesium, and silicon are important components of human bone. The successful biomimicking of human bone characteristics involves incorporating all the human bone elements into the scaffold material.

Exploring temperature dynamics in Madhya Pradesh: a spatial-temporal analysis.

Understanding the dynamics of temperature trends is vital for assessing the impacts of climate change on a regional scale. In this context, the present study focuses on Madhya Pradesh state in Central Indian region to explore the spatial-temporal distribution patterns of temperature changes from 1951 to 2021. Gridded temperature data obtained from the Indian Meteorological Department (IMD) in 1° × 1° across the state are utilised to analyse long-term trends and variations in temperature.

Design and Development of IoT and Deep Ensemble Learning Based Model for Disease Monitoring and Prediction.

With the rapidly increasing reliance on advances in IoT, we persist towards pushing technology to new heights. From ordering food online to gene editing-based personalized healthcare, disruptive technologies like ML and AI continue to grow beyond our wildest dreams. Early detection and treatment through AI-assisted diagnostic models have outperformed human intelligence.

Stability-Indicating HPTLC Method for Determination of Remogliflozin Etabonate and Vildagliptin in Tablets.

A sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (HPTLC) method is developed for the simultaneous estimation of remogliflozin etabonate and vildagliptin in the presence of their degradation products. The separation was performed in thin layer chromatography plate precoated with silica gel G60F254. The mobile phase consists of methanol:ethyl acetate:toluene:ammonia (1.

Review of Physical, Mechanical, and Biological Characteristics of 3D-Printed Bioceramic Scaffolds for Bone Tissue Engineering Applications.

This review focuses on the advancements in additive manufacturing techniques that are utilized for fabricating bioceramic scaffolds and their characterizations leading to bone tissue regeneration. Bioscaffolds are made by mimicking the human bone structure, material composition, and properties. Calcium phosphate apatite materials are the most commonly used scaffold materials as they closely resemble live bone in their inorganic composition.

Modulation of the structural information in shape-defined heterocyclic strands: the case of a (pyridine-hydrazone)pyrazine ligand.

Metal ions (Ag, Cd, Eu, Sm) and protons can, through coordination and protonation, modulate in three specific ways the structural information contained in the pyrazine-based heterocyclic strand L (obtained from 2,5-bis(methylhydrazino)pyrazine and 2 equivalents of 2-pyridinecarboxaldehyde), thus generating two linear rod-like conformations and a bent one. This conformational diversity is associated with a structural one that consists of two diprotonated forms (HL(PF) and HL(CFSO)), a polymeric architecture [AgL](CFSO), two rack-like complexes ([EuHL(CFSO)](PF) and [SmHL(CFSO)](PF)) and a grid-like structure ([CdL](CFSO)).

Comprehensive Review on Design and Manufacturing of Bio-scaffolds for Bone Reconstruction.

Bio-scaffolds are synthetic entities widely employed in bone and soft-tissue regeneration applications. These bio-scaffolds are applied to the defect site to provide support and favor cell attachment and growth, thereby enhancing the regeneration of the defective site. The progressive research in bio-scaffold fabrication has led to identification of biocompatible and mechanically stable materials.

Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes: A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia.

The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (Re ) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, Re -diimine complex already exhibits excellent emission yield (34%, λ   = 583 nm) and large two-photon absorption cross-sections (σ   = 300 GM @ 800 nm) in water (pH 7.

A Survey on Security and Privacy Issues in Contact Tracing Application of Covid-19.

In response to the coronavirus (COVID-19) pandemic, Government and public health authorities around the world are developing contact tracing apps as a way to trace and slow the unfold of the virus. There is major divergence among nations, however, between a "privacy-first" approach that protects citizens' information at the price of very restricted access for public health authorities and a "data-first" approach that stores massive amounts of knowledge that, whereas of immeasurable price to epidemiologists. Contact tracing apps work by gathering information from people who have tested positive for the virus and so locating and notifying individuals with whom those people are in shut contact, oftentimes by use of GPS, Bluetooth, or wireless technology.

Extraarticular extrasynovial solitary osteochondromatosis of the ankle: A rare case report and review of literature.

Introduction: Synovial chondromatosis (SC) is a relatively common benign condition of the synovial joint characterized by the formation of cartilaginous nodules in synovium. However, extra articular osteochondromatosis is rare and only few have been reported around the ankle joint. We have reported such a presentation and have reviewed the literature extensively.

Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1.

The isolation, screening, and identification of multi-metal resistant (Cd, Cu, Pb, and Zn) bacteria from polluted coastal sediment samples were performed. In this study, the isolates S2-2 and S3-2 had higher multi-metal resistance and were identified as Pseudomonas pachastrellae KMS2-2 and Bacillus cereus KMS3-1, respectively. One-variable-at-a time approach suggested that optimum conditions for exopolysaccharides (EPS) production were pH 7.

Improving the Accuracy of Feature Selection in Big Data Mining Using Accelerated Flower Pollination (AFP) Algorithm.

In recent times, the main problem associated with big data analytics is its high dimensional data over the search space. Such data gathers continuously in search space making traditional algorithms infeasible for data mining in real time environment. Hence, feature selection is an important method to lighten the load during processing while inducing a model for mining.

A new approach to classify and rank events based videos based on Event of Detection.

In the ongoing days, the development of sight and sound substance and information stockpiling produces colossally. Clients can extricate any kind of data they require from recordings. This outcomes in quick development of video information, client's discover complexity while procurement their important data.

Proteomic analysis of rat prefrontal cortex after chronic valproate treatment.

Valproic acid (VPA) is commonly used to treat bipolar disorder (BD), but its therapeutic role has not been clearly elucidated. To gain insights into VPA's mechanism of action, proteomic analysis was used to identify differentially expressed proteins in the rat prefrontal cortex (PFC), a region particularly affected in BD, after 6 weeks of VPA treatment. Proteins from PFCs of control and VPA-treated rats were separated by 2D-DIGE and identified by mass spectrometry.

Rat brain myo-inositol 3-phosphate synthase is a phosphoprotein.

The therapeutic effects of lithium in bipolar disorder are poorly understood. Lithium decreases free inositol levels by inhibiting inositol monophosphatase 1 and myo-inositol 3-phosphate synthase (IPS). In this study, we demonstrate for the first time that IPS can be phosphorylated.

Linking tRNA localization with activation of nutritional stress responses.

Cells respond to nutrient deprivation a variety of ways. In addition to global downregulation of cap-dependent protein synthesis mediated by the GCN2 and mTORC1 signaling pathways, a catabolic process autophagy is upregulated to provide internal building blocks and energy needed to sustain viability. It has recently been shown that during nutrient deprivation tRNAs accumulate in the nucleus, but the functional role of this accumulation remains unknown.

Fibroblast-specific expression of AC6 enhances beta-adrenergic and prostacyclin signaling and blunts bleomycin-induced pulmonary fibrosis.

Pulmonary fibroblasts regulate extracellular matrix production and degradation and are critical in maintenance of lung structure, function, and repair, but they also play a central role in lung fibrosis. cAMP-elevating agents inhibit cytokine- and growth factor-stimulated myofibroblast differentiation and collagen synthesis in pulmonary fibroblasts. In the present study, we overexpressed adenylyl cyclase 6 (AC6) in pulmonary fibroblasts and measured cAMP production and collagen synthesis.

CD82 endocytosis and cholesterol-dependent reorganization of tetraspanin webs and lipid rafts.

Tetraspanin CD82 suppresses cell migration, tumor invasion, and tumor metastasis. To determine the mechanism by which CD82 inhibits motility, most studies have focused on the cell surface CD82, which forms tetraspanin-enriched microdomains (TEMs) with other transmembrane proteins, such as integrins. In this study, we found that CD82 undergoes endocytosis and traffics to endosomes and lysosomes.

The C1 and C2 domains target human type 6 adenylyl cyclase to lipid rafts and caveolae.

Previous data has shown that adenylyl cyclase type 6 (AC6) is expressed principally in lipid rafts or caveolae of cardiac myocytes and other cell types while certain other isoforms of AC are excluded from these microdomains. The mechanism by which AC6 is localized to lipid rafts or caveolae is unknown. In this study, we show AC6 is localized in lipid rafts of COS-7 cells (expressing caveolin-1) and in HEK-293 cells or cardiac fibroblasts isolated from caveolin-1 knock-out mice (both of which lack prototypical caveolins).

Adenylyl cyclase type 6 overexpression selectively enhances beta-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts.

Cardiac fibroblasts produce and degrade extracellular matrix and are critical in regulating cardiac remodeling and hypertrophy. Fibroblasts are activated by factors such as transforming growth factor beta and inhibited by agents that elevate 3',5'-cyclic adenosine monophosphate (cAMP) levels. cAMP signal generation and response is known to be compartmentalized in many cell types in part through the colocalization of receptors and specific adenylyl cyclase isoforms in lipid rafts and caveolae.

A comparative study on the effect of plant extracts with the antibiotics on organisms of hospital origin.

Thirty five plants belonging to twenty families were studied for their antimicrobial activity. Among the plants tested, 43 % showed antimicrobial activity. Fifteen plants belonging to 10 families exhibited activity against gram positive bacteria and gram negative bacteria.

Epidermal growth factor prevents acetaldehyde-induced paracellular permeability in Caco-2 cell monolayer.

Background: Intestinal permeability and endotoxemia play a crucial role in the pathogenesis of alcoholic liver disease. Previous studies showed that acetaldehyde disrupts intestinal epithelial barrier function and increases paracellular permeability by a tyrosine kinase-dependent mechanism. In the present study, the role of epidermal growth factor (EGF) in protection of epithelial barrier function from acetaldehyde was evaluated in Caco-2 intestinal epithelial cell monolayer.

Dietary influence of selenium on the incidence of N-nitrosodiethylamine-induced hepatoma with reference to drug and glutathione metabolizing enzymes.

The dietary administration of selenium (sodium selenite; 4 p.p.m.

Ocular manifestations of the lacrimo-auriculo-dento-digital syndrome.

We studied a mother and daughter with an extremely rare constellation of signs and symptoms. One or both had absent lacrimal puncta, nasolacrimal duct obstruction, chronic dacryocystitis, dry eyes, and epiphora. Systemic findings included salivary gland hyposecretion, dental hypoplasia and dysplasia, cup-shaped ears with hearing loss, and digital anomalies.