The urinary cotinine and serum 25 hydroxyvitamin D levels in male smokers.

Objective: To assess urinary cotinine and the effects of smoking on 25(OH)D levels in 67 male smokers.

Material And Method: Urine and blood specimens were analyzed for cotinine and serum 25 hydroxyvitamin D (25(OH)D) concentrations by high performance liquid chromatography (HPLC) and chemiluminescent immunoassay, respectively. The accuracy, precision and detection limit of the HPLC method were also tested.