Publications by authors named "Thanaporn Chuen-Im"
<b>Background and Objective:</b> <i>Eurycoma longifolia</i> roots hold traditional medicinal value, but scientific evaluation of their bioactivity and safety is lacking. This study investigated the antioxidant and anticancer potential of <i>E. longifolia</i> extracts and assessed cytotoxicity.
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- - The study focused on a bacterial strain ZO16 from ginger roots, identified as *Streptomyces prasinus*, aiming to isolate and analyze its bioactive compounds for antibacterial and anticancer properties.
- - Researchers used techniques like chromatography and spectroscopy to purify compounds, identifying them as endophenazine A and B, which showed antibacterial effects against harmful bacteria and varying effectiveness against cancer cells.
- - The isolated compounds had a minimum inhibitory concentration of 8 to 32 μg/mL against bacteria and associated IC50 values for different cancer cells, but they also exhibited moderate toxicity to non-cancerous cells, suggesting potential but necessitating further research.
Human papillomaviruses (HPVs) infect cutaneous and mucosal epithelia to cause benign (warts) and malignant lesions (e.g. cervical cancer).
View Article and Find Full Text PDF<b>Background and Objective:</b> The RH3.5 was isolated from the rhizosphere of <i>Boesenbergia rotunda</i> (L.) Mansf.
View Article and Find Full Text PDF<b>Background and Objective:</b> The SU84 was isolated from the rhizosphere of <i>Curcuma longa</i> and identified to be <i>Streptomyces</i> sp. via analysis of its 16S rDNA sequence, chemotaxonomy and morphology. This study aimed to isolate major compounds from the extract culture of strain SU84 and evaluate their antibacterial activity.
View Article and Find Full Text PDFBackground/aim: Tetrazolium-based cell proliferation assays using MDA-MB-231 and HeLa cells revealed that 3,4-dihydro-lactucin (3,4-DHL), a compound isolated from Microbispora rosea AL22, possesses anticancer properties. Apoptotic cell death was observed in 3,4-DHL-treated cells. Lactucopicrin, a related compound, reportedly exerts anticancer activity against different cancer types.
View Article and Find Full Text PDFCervical cancer screening typically involves a Pap smear combined with high-risk human papillomavirus (hr-HPV) detection. Women with hr-HPV positivity but normal cytology, as well as those with precancerous abnormal cytology, such as low-grade squamous intraepithelial lesions (LSIL) and high-grade SIL (HSIL), are referred for colposcopy and histology examination to identify abnormal lesions, such as cervical intraepithelial neoplasia (CIN) and cervical cancer. However, in order to enhance the accuracy of detection, bioinformatics analysis of a microarray database was performed, which identified cg01009664, a methylation marker of the thyrotropin-releasing hormone (TRH).
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- - The AL22 strain of *Microbispora* sp. was isolated from the rhizosphere of *Alpinia galanga* and found to have antibacterial properties against various pathogens, prompting further investigation into its major compounds and their biological effects.
- - Two key compounds, 3,4-dihydro-lactucin and umbelliferone, were identified through chromatography and characterized using NMR and mass spectrometry, with 3,4-dihydro-lactucin demonstrating significant antibacterial activity against gram-positive bacteria.
- - While the crude extract and identified compounds showed low toxicity in normal cell lines, 3,4-dihydro-lactucin exhibited cytotoxic effects on certain cancer cell lines, indicating its potential as
<b>Background and Objective:</b> Synergistic combinations of antimicrobial agents with different mechanisms of action are successful approaches for combating bacterial infections. This study aimed to evaluate the synergistic effect of 1-methyl ester-nigericin <b>(1)</b> and methyl 5-(hydroxymethyl) furan-2-carboxylate <b>(2)</b> against <i>Proteus</i> spp., isolates.
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- Antibiotic resistance in microorganisms poses a significant health risk to both humans and animals, contributing to treatment failures and higher mortality rates from infections.
- Research at the Sea Turtle Conservation Center of Thailand revealed high mortality rates among juvenile turtles due to bacterial infections, with a connection found between bacterial presence in holding tank seawater and infections in the turtles.
- The study spanning from 2015 to 2019 found that a majority of bacterial isolates in the seawater were resistant to at least one antibiotic, highlighting the growing concern of antibiotic-resistant bacteria and their impact on the health of sea turtles in captivity.
In this study, levels of Vibrionaceae, Staphylococcaceae, and Enterobacteriaceae were observed in seawater from juvenile green turtle Chelonia mydas rearing tanks and in the incoming coastal seawater (the water supply). Bacterial loads were compared between the incoming coastal seawater and two different rearing conditions: in cement tanks at a low stocking density and in fiberglass tanks at a high stocking density. The total bacterial counts in seawater from fiberglass tanks were statistically greater than those in cement tanks.
View Article and Find Full Text PDFInhibition of primary oocyte developing to secondary oocyte results from oocyte maturation inhibitor (OMI) which is secreted from oocyte-surrounding cumulus cells (CCs) during forming complexes with an oocyte. Development of primary oocyte occurs when the CCs are dissociated from the oocyte. This research studied the effects of pFSH, LH, and estradiol supplementation in culture medium on ultrastructures of porcine cumulus oocyte complexes (pCOCs) using transmission electron microcopy (TEM) and inverted microscopy.
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- Human papillomaviruses (HPVs) types 16 and 18 are the main culprits behind cervical disease, with the E2 transcription factor playing a key role in regulating viral and cellular gene expression.
- Research highlights how high-risk HPV E2 enhances the production of the oncoprotein SF2/ASF, which is crucial for RNA splicing, indicating that dysregulation of SR proteins like SF2/ASF may contribute to diseases such as cancer.
- The study also shows that in HPV16-infected cells, specific SR proteins are up-regulated during cervical tumor progression and may serve as potential biomarkers for HPV-related diseases.