Prediction of the postharvest quality of Boletus wild mushrooms stored with mesoporous silica nanoparticles antibacterial film using Long Short-Term Memory model combined with the Northern Goshawk Optimization (NGO-LSTM).

This study aimed to address the challenge of extending the shelf life of Boletus wild mushrooms, which are prone to environmental and microbial contamination. An antibacterial film composed of polylactic acid (PLA) and mesoporous silica nanoparticles loaded with citral (CMP film) was developed for this purpose. Fifteen quality indices were assessed, and the data were integrated using AHP and TOPSIS to evaluate the film's efficacy.

A pH-sensitive film based on chitosan/gelatin and anthocyanin from Diels for monitoring fish freshness.

As a new type of packaging method, the anthocyanin-based pH-sensitive indicator film has gained much attention owing to low cost, small size, and visually informative property. In this study, an intelligent film based on chitosan/gelatin (CG) matrix with Diels (ZSD) anthocyanin for fish freshness monitoring was developed. The film properties, including thickness, moisture content, color, mechanical properties, UV-vis light barrier property, as well as pH and ammonia sensitivity, were evaluated.

Invited review: Role of Bacillus licheniformis in the dairy industry-Friends or foes?

Bacillus licheniformis is one of the major spore-forming bacteria with great genotypic diversity in raw milk, dairy ingredients, and final dairy products; it is found throughout the dairy-processing continuum. Although being widely used as a probiotic strain, this species also serves as a potential risk in the dairy industry based on its roles in foodborne illness and dairy spoilage. Biofilm formation of B.

Cell-wall-anchored proteins affect invasive host colonization and biofilm formation in Staphylococcus aureus.

As a major human and animal pathogen, Staphylococcus aureus can attach to medical implants (abiotic surface) or host tissues (biotic surface), and further establish robust biofilms which enhances resistance and persistence to host immune system and antibiotics. Cell-wall-anchored proteins (CWAPs) covalently link to peptidoglycan, and largely facilitate the colonization of S. aureus on various surfaces (including adhesion and biofilm formation) and invasion into host cells (including adhesion, immune evasion, iron acquisition and biofilm formation).

Controlling release of astaxanthin in β-sitosterol oleogel-based emulsions via different self-assembled mechanisms and composition of the oleogelators.

In this study, three types of β-sitosterol-based oleogels (β-sitosterol + γ-oryzanol oleogels, β-sitosterol + lecithin, oleogels and β-sitosterol + monostearate oleogels), loaded with astaxanthin, were employed as the oil phase to create oleogel-based emulsions (SO, SL, and SM) using high-pressure homogenization. The microstructure revealed that fine-scale crystals were dispersed within the oil phase of the droplets in the β-sitosterol oleogel-based emulsion. The bioaccessibility of astaxanthin was found to be 58.

Differential alteration in subsp. quorum-sensing systems and reduced yeast survival and virulence gene expression in dual-species interaction.

() and subsp. () are frequently identified in various niches, but their dual-species interaction, especially with in yeast form, remains unclear. This study aimed to investigate the dual-species interaction of and , including proliferation, morphology, and transcriptomes examined by selective agar plate counting, microscopy, and polymicrobial RNA-seq, respectively.

Sub-MIC streptomycin and tetracycline enhanced Guangzhou-SAU749 biofilm formation, an in-depth study on transcriptomics.

is a major human pathogen, a potential "Super-bug" and a typical biofilm forming bacteria. With usage of large amount of antibiotics, the residual antibiotics in clinical settings further complicate the colonization, pathogenesis and resistance of . This study aimed at investigating the phenotypical and global gene expression changes on biofilm formation of a clinical isolate treated under different types of antibiotics.

Antimicrobial Resistance, SCC, Virulence and Genotypes of MRSA in Southern China for 7 Years: Filling the Gap of Molecular Epidemiology.

As the prevalence of infections is of worldwide concern, phenotype and genotype in prevalent MRSA strains require longitudinal investigation. In this study, the antibiotic resistance, virulence gene acquisition, and molecular type were determined on a large scale of nosocomial strains in Southern China during 2009-2015. Bacterial identification and antimicrobial susceptibility to 10 antibiotics were tested by Vitek-2.

Microbial Interaction between Lactiplantibacillus plantarum and Saccharomyces cerevisiae: Transcriptome Level Mechanism of Cell-Cell Antagonism.

Lactiplantibacillus plantarum and Saccharomyces cerevisiae are frequently co-isolated in food, although playing different roles. This study aimed at investigating the microbial interaction between L. plantarum and S.

Verification and application of a modified carbapenem inactivation method (mCIM) on : a potential screening methodology on carbapenemases phenotype in .

Antimicrobial resistance (AMR) has been a leading issue for human health globally threatening the achievement of several of the Sustainable Development Goals (SDGs). Originated from , carbapenemases phenotype has been considered to be a major concern in AMR. In this study, the AMR identification rate of isolates and infections in FAHJU showed an obvious upward trend from 2012 to 2016.

Establishment and application of a rapid visual detection method for based on polymerase spiral reaction (PSR).

is a common foodborne pathogen that presents in various food products, posing important threat to public health. The aim of this study was to establish a rapid and sensitive method with visualization to detect based on polymerase spiral reaction (PSR). Primers targeting conserved gene sequence of were designed based on bioinformatics analyses on the current available genomes.

A strategy design based on antibiotic‑resistance and plasmid replicons genes of clinical Escherichia coli strains.

Since antimicrobial resistance, especially β-lactam resistance genes were common in clinical strains, this study had designed and developed multiplex amplification platform for rapid and accurate detection of such resistance genes in 542 clinical isolates. The obtained specimens were subjected to bacteriological examination, antimicrobial susceptibility testing, and detection of β-lactamase genes and plasmid replicons. The major virulence genes were detected by 7 groups of multiplex PCR and eight groups of multiplex PCR were designed to detect 8 different plasmid replicons including , iteron, , and .

Regulatory network controls microbial biofilm development, with as a representative: from adhesion to dispersal.

Microorganisms mainly exist in the form of biofilm in nature. Biofilm can contaminate food and drinking water system, as well as cause chronic wound infections, thereby posing a potential threat to public health safety. In the last two decades, researchers have made efforts to investigate the genetic contributors control different stages of biofilm development (adherence, initiation, maturation, and dispersal).

Significant downtrend of antimicrobial resistance rate and rare β-lactamase genes and plasmid replicons carriage in clinical Pseudomonas aeruginosa in Southern China.

Objectives: Pseudomonas aeruginosa is a medically important pathogen showing intrinsic low permeability to various antimicrobial agents and its potential to acquire multiple resistance mechanism. A longitudinal surveillance aimed to investigate the antimicrobial resistance and its determinants of Pseudomonas aeruginosa in Southern China. A total of 2163 P.

Impact of pmrA on Cronobacter sakazakii planktonic and biofilm cells: A comprehensive transcriptomic study.

Cronobacter sakazakii is an emerging opportunistic foodborne pathogen causing rare but severe infections in neonates. Furthermore, the formation of biofilm allows C. sakazakii to persist in different environments.

Polymicrobial interaction between and : coexistence-relevant mechanisms.

The coordination of single or multiple microorganisms are required for the manufacture of traditional fermented foods, improving the flavour and nutrition of the food materials. However, both the additional economic benefits and safety concerns have been raised by microbiotas in fermented products. Among the fermented products, and are one of the stable microbiotas, suggesting their interaction is mediated by coexistence-relevant mechanisms and prevent to be excluded by other microbial species.

Antimicrobial susceptibility and genetic features of a heterogeneous vancomycin intermediate-resistant Staphylococcus aureus strain.

This study aimed to characterize the antimicrobial susceptibility and genetic features of a heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) strain Guangzhou-SauVS2 recovered from a female patient in Guangzhou, representative of southern China. The genome of Guangzhou-SauVS2 was sequenced using Illumina HiSeq 2500 platform and assembled de novo using Velvet v1.2.

Resistome and virulome study on pathogenic Streptococcus agalactiae Guangzhou-SAG036.

Streptococcus agalactiae is considered as a leading case of bacterial infection among neonates. Although relative protection strategies have been performed in many high-income countries, resulting in a massive reduction in the occurrences of early-onset GBS disease, the late-onset disease has not affected. Here, the whole genome of S.

Genomic analysis of a hop-resistance Lactobacillus brevis strain responsible for food spoilage and capable of entering into the VBNC state.

Background: Lactobacillus brevis is a major contaminant of spoiled beer. And it was able to enter VBNC state and cause false negative detection, which poses a major challenge to the brewing industry.

Methods: The genomic DNA of L.

Spoilage Lactic Acid Bacteria in the Brewing Industry.

Lactic acid bacteria (LAB) have caused many microbiological incidents in the brewing industry, resulting in severe economic loss. Meanwhile, traditional culturing method for detecting LAB are time-consuming for brewers. The present review introduces LAB as spoilage microbes in daily life, with focus on LAB in the brewing industry, targeting at the spoilage mechanism of LAB in brewing industry including the special metabolisms, the exist of the viable but nonculturable (VBNC) state and the hop resistance.

Inhibitory effects of two types of food additives on biofilm formation by foodborne pathogens.

The inhibition of microbial biofilms is a significant concern in food safety. In the present study, the inhibitory effect of sodium citrate and cinnamic aldehyde on biofilm formation at minimum inhibitory concentrations (MICs) and sub-MICs was investigated for Escherichia coli O157:H7 and Staphylococcus aureus. The biofilm inhibition rate was measured to evaluate the effect of sodium citrate on S.

Protective effects of polyphenolic extracts from longan seeds promote healing of deep second-degree burn in mice.

The objective of this study was to evaluate the ability of a polyphenolic extract from longan seeds as a wound-healing material for deep second-degree burn wounds. The total phenol content of the extract reached 45.28 ± 1.