Gelatin capsules for post mortem vitrification of Piracanjuba (Brycon orbignyanus) ovarian tissue as an accessible solution for emergency genetic preservation.

Environmental disasters have caused significant losses in fish populations, threatening the genetic diversity of many species. Cryopreservation of genetic material post-mortem has emerged as a potential solution to safeguard biodiversity. However, a fast, accessible method for cryopreserving such material in emergencies is needed.

Biodegradable capsules as a sustainable and accessible container for vitrification of gonadal tissue using the zebrafish animal model.

Cryopreservation of fish gonadal tissue is an important technique for preserving genetic variability. However, this technique involves the use of cryotubes, plastic containers with low degradability that are expensive and difficult to obtain in certain parts of the world. Therefore, this study aimed to evaluate the efficiency of gelatin and hypromellose hard capsules as a sustainable and accessible alternative container to the cryotube for vitrification of zebrafish (Danio rerio) gonadal tissue.

Fish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology.

In Brief: The containers used in cell cryopreservation are essential to maintain cell integrity and viability after thawing. This paper reveals the methodology of using biodegradable containers for fish sperm cryopreservation. Cryopreserved sperm in biodegradable containers showed high fertility capability.

Hydrogel encapsulation as a handling and vitrification tool for zebrafish ovarian tissue.

Zebrafish is an important animal model, thousands lines have been developed, thus having a great need for their preservation. However, the cryopreservation of fish oocytes is still limited and needs improvement. The sodium alginate hydrogel, in addition to providing support for the cells, has been shown to be a potential cryoprotectant.