Co-occurrence of blaNDM-1 and blaOXA-23 in carbapenemase-producing Acinetobacter baumannii belonging to high-risk lineages isolated from burn patients in Tunisia.

Aims: Carbapenem-resistant Acinetobacter baumannii (CR-Ab) is an important cause of infections in burn patients. This study aimed to characterize the antimicrobial susceptibility pattern of CR-Ab isolated from burns in Burn Intensive Care Unit (BICU) of the Trauma and Burn Centre of Ben Arous, to determine the prevalence of β-lactamase-encoding genes and to search eventual genetic relatedness of CR-Ab strains.

Methods And Results: From 15 December 2016 to 2 April 2017, all nonduplicated CR-Ab isolated in burn patients in the BICU were screened by simplex Polymerase Chain Reaction (PCR) for the class A, B, C, and D β-lactamase genes.

Detection of carbapenem resistant Pseudomonas aeruginosa co-harboring blaVIM-2 and blaGES-5 in burn patients.

Pseudomonas aeruginosa is one of the major infectious agents in burn patients. Globally, high rates of antimicrobial resistance in P. aeruginosa have been reported, which is a cause of concern.

Methicillin-Resistant Strains Isolated from Burned Patients in a Tunisian Hospital: Molecular Typing, Virulence Genes, and Antimicrobial Resistance.

Methicillin-resistant (MRSA) is one of the major causes of a variety of infections in hospitals and the community. Their spread poses a serious public health problem worldwide. Nevertheless, in Tunisia and other African countries, very little molecular typing data on MRSA strains is currently available.

Widespread of the Vienna/Hungarian/Brazilian CC8-ST239-SCCmec III MRSA clone in patients hospitalized in the Tunisian Burn and Traumatology Center.

The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals is a major global public health concern. The current study sought to characterize 25 MRSA clinical isolates collected in a Tunisian hospital from December 2015 to September 2016, with the genetic lineages, virulence factors, and antibiotic resistance mechanisms determined for these isolates. Three spa-types were detected: t037 (23 isolates), t932, and t2235 (one isolate each).

Dissemination of epidemic ST239/ST241-t037-agrI-SCCmecIII methicillin-resistant Staphylococcus aureus in a Tunisian trauma burn intensive care unit.

Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen causing health care-infections in the world, especially in burns. The aim of this study was to assess the extent of dissemination of MRSA isolated from burn patients in Burn Intensive Care Unit in Tunisia and to evaluate the frequency of virulence and antibiotics resistance genes. Among the 72 S.

Rapid diagnostic tests : Pros, cons and potential use in the COVID-19 management in Tunisia.

Objectives: Diagnosis of SARS-CoV-2 infection is a major public health issue. In a context of limited diagnostic capacity with the reference technique (real-time RT-PCR), many manufacturers have developed rapid diagnostic tests (RDTs). Although very promising in theory, these tests have raised many questions.

Molecular profile of carbapenemase-producing Enterobacterales in burn patients.

Background: Carbapenemase-producing Enterobacterales (CPE) present a threat to public health worldwide.

Aim: To study their prevalence at the Trauma and Burn Center's Burn Unit and investigate their molecular characteristics and their associated antibiotics resistance patterns.

Methods: This is a retrospective study conducted at the Trauma and Burn Center's laboratory between july 2017 and december 2018.

Clonal spread of PER-1 and OXA-23 producing extensively drug resistant Acinetobacter baumannii during an outbreak in a burn intensive care unit in Tunisia.

Extensively drug resistant Acinetobacter baumannii (XDR-Ab), has emerged as an important pathogen in several outbreaks. The aim of our study was to investigate the eventual genetic relatedness of XDR-Ab strains recovered from burn patients and environment sites in the largest Tunisian Burn Intensive Care Unit (BICU) and to characterize β-lactamase encoding genes in these strains. Between March 04th, 2019 and April 22nd, 2019 an outbreak of XDR-Ab was suspected.

SARS-CoV-2 infection virological diagnosis.

SARS-CoV-2 infection has to be confirmed by virological diagnosis. Multiple diagnostic tests are available without enough perspective on their reliability. Therefore, it is important to choose the most suitable test according to its sensitivity and specificity but also to the stage of the disease.

Correction to: P-42 Distribution and antifungal susceptibility of Candida isolates in a Tunisian burn unit.

Burned patients are at high risk of yeast colonization and thus of invasive fungal infections, particularly to Candida (C.) spp., leading to an increase in morbidity and mortality.

[Comparative study of antibiotic resistance in bacteria isolated from burned patients during two periods (2005-2008, 2008-2011) and in two hospitals (Hospital Aziza Othmana, Trauma and Burn Center)].

Background: Continuous monitoring of the bacterial flora and antibiotic resistance of the main bacteria involved in nosocomial infections helps improve treatment and prevention strategies.

Aim: To compare the bacteriological profile and antibiotic susceptibility of the main bacterial isolates within the burned patients over two periods of 3 years and in two hospitals.

Methods: During two three-year periods: period 1 (P1): 1/7/2005-30/6/2008 and period 2 (P2): 1/7/2008-30/6/2011) and in two hospitals: Hospital Aziza Othmana (HAO) and the traumatology and burn center (CTGB), 2153 and 3719 non-repetitive strains were isolated from burn patients from different samples.

Epidemiological profile and antibiotic resistance of Pseudomonas aeruginosa isolates in burn and Traumatology center in Tunisia over a three-year period.

Background: Pseudomonas aeruginosa is a known opportunistic pathogen frequently causing serious infections in burned patients.

Aim: To analyze the epidemiological profile of Pseudomonas aeruginosa isolated in a Tunisian burn unit.

Methods: During a 3-year period (from 01 July 2008 to 30 June 2011), 544 non repetitive strains of P.

[Bacteriological profile of urinary tract infections in women in Aziza Othmana Hospital: 495 cases].

Background: Urinary infection is a frequent pathology in the community as well as at the hospital.

Aim: To analyze the profile of bacteria isolated from urinary tract infectious in women and their antimicrobial resistance.

Methods: During two year period (1 January 2005 to 31 December 2006), 4536 urinary specimens were analyzed at the Laboratory of Aziza Othmana Hospital.

The impact of fluoroquinolones use on antibiotic resistance in an intensive care burn department.

Background: Fluoroquinolones (FQ) use has been identified as a risk factor for colonization and infection to methicillin resistant Staphylococcus aureus(MRSA), Pseudomonas aeruginosae multiresistant(PMR), Acinetobacter multiresistant (AMR) and multidrug resistant bacteria(MDRB).

Aim: Our study proposes to measure the annual antibiotic use of FQ and antimicrobial resistance in P. aeruginosa, S.

The impact of fluoroquinolones use on antibiotic resistance in an intensive care burn department.

Background: Fluoroquinolones (FQ) use has been identified as a risk factor for colonization and infection to methicillin resistant Staphylococcus aureus(MRSA), Pseudomonas aeruginosae multiresistant(PMR) , Acinetobacter multiresistant (AMR) and multidrug resistant bacteria(MDRB).

Aim: Our study proposes to measure the annual antibiotic use of FQ and antimicrobial resistance in P. aeruginosa, S.

[Bacteriological profile and antibiotic resistance of bacteria isolates in a burn department].

Background: Nosocomial infections remain the main cause of morbidity and mortality in burn patients. Ongoing surveillance of infections in burned patients is essential to detect changes in epidemiology and to guide better empirical antibiotherapy and infection control policies. The aim of this study is to analyze the bacterial flora and the antibiotic resistance of isolates in a burn department during a two year period

Methods: From 1 January 2005 to 31 December 2006, 1268 strains were isolated from different specimens.

[Surveillance of multidrug resistant bacteria in a Tunisian hospital].

Background: Controlling antibiotic resistance of bacteria is a priority for public healthcare.

Aim: This study concerned the frequency of multidrug resistant bacteria (MDRB) in a Tunisian Hospital with the aim of establishing guidelines for MDRB prevention.

Methods: The study was conducted during two years (1 January 2005-31 December 2006).

[Evolution of antibiotic consumption in an intensive care burn department].

Background: The increasing consumption of antibiotics in hospitals and the economic implications of this increase lead to survey this consumption in the various hospital units.

Aim: Our study proposes to measure the annual antibiotic use and antimicrobial resistance in an intensive care Burn department in order to manage the control measures.

Methods: The study was conducted during a 5 year period (1 January 2000 to 31 December 2004).

Association between antibiotic use and changes in susceptibility patterns of Pseudomonas aeruginosa in an intensive care burn unit: a 5-year study, 2000-2004.

This study aimed to evaluate the relationship between antimicrobial resistance in Pseudomonas aeruginosa and annual antibiotic use in a burn unit. From 1 January 2000 to 31 December 2004, 203 non-repetitive strains of Ps. aeruginosa were recovered from various clinical specimens.

[Epidemiological profile and antibiotic susceptibility of Pseudomonas aeruginosa isolates within the burned patient hospitalized in the intensive care burn unit].

Background: Pseudomonas aeruginosa plays a predominant role as an etiological agent involved in serious infections in burned patients. Treatment of these infections is frequently complicated by antibiotic resistance, a problem that is is increasing in recent years.

Aim: The objective of this study is to analyze epidemiological profile and antibiotic susceptibility of P.

[A study of sensitivity to antibiotics for Staphylococcus aureus strains isolated in a department of resuscitation of badly burnt patients].

Methicillin resistant Staphylococcus aureus (MRSA) constitutes one of the main causes of nosocomial infections in badly burnt patients. The purpose of our study was to determine the frequency and evolution of Methicillin resistant Staphylococcus strains in the department of resuscitation of badly burnt patients of Hopital AZIZA OTHMANA. From January 1, 2000 to December 31, 2003, tests for Staphylococcus aureus proved positive in 139 patients on at least, one occasion.

[Bacteriological profile of bacteremias in badly burnt patients].

The purpose of this work is to study the bacteriological profiles of germ responsible for bacteriemas / septicemias in badly burnt patients hospitalized in burns utis, in order to guide better the antibiotherapy of first intention. During the period of survey going from January 1st, 2001 to December 31st,2002 71 patients had one or several episodes of bateremia. The average burnt cutaneous surface was 48.

[Pseudomonas aeruginosa: a multicentric study of antibiotic resistance (1999-2000)].

A retrospective multicentric study was carried out over a period of 2 years (1999-2000). 2659 strains of Pseudomonas aeruginosa were collected from 4 university hospitals (Charles Nicolle Hospital, Pediatric Hospital and National Centre of Bone Marrow Transplantation in Tunis, Habib Bourguiba Hospital in Sfax). Epidemiological profile and antibiotic susceptibility were analysed.

[Antibiotic resistance among E. coli isolates from urinary tract infections (1999-2000): a multicenter study].

E. coli is the most frequently isolate species in community as well as in nosocomial acquired urinary tract infections (UTI). Trimethoprim-sulfamethoxazole (TMP-SMX), is commonly used as empiric treatment for managing uncomplicated UTI whereas the rate of resistance to amoxicilline is increasing.