Evaluation of a blood miRNA/mRNA signature to follow-up Lu-PRRT therapy for G1/G2 intestinal neuroendocrine tumors.

Background: Lu-oxodotreotide peptide receptor therapy (LuPRRT) is an efficient treatment for midgut neuroendocrine tumors (NETs) of variable radiological response. Several clinical, biological, and imaging parameters may be used to establish a relative disease prognosis but none is able to predict early efficacy or toxicities. We investigated expression levels for mRNA and miRNA involved in radiosensitivity and tumor progression searching for correlations related to patient outcome during LuPRRT therapy.

Impact of different models based on blood samples and images for bone marrow dosimetry after Lu-labeled somatostatin-receptor therapy.

Background: Peptide receptor radionuclide therapy with Lu-DOTATATE is a recognized option for treating neuroendocrine tumors and has few toxicities, except for the kidneys and bone marrow. The bone marrow dose is generally derived from a SPECT/CT image-based method with four timepoints or from a blood-based method with up to 9 timepoints, but there is still no reference method. This retrospective single-center study on the same cohort of patients compared the calculated bone marrow dose administered with both methods using mono, bi- or tri-exponential models.

Impact of radiation on host immune system in patients treated with chemoradiotherapy and durvalumab consolidation for unresectable locally advanced non-small cell lung cancer.

Background: The optimal modalities of radiotherapy when combining concurrent chemoradiation (CCRT) and immunotherapy (IO) for locally advanced non-small cell lung cancer (LA-NSCLC) remain to be determined. The aim of this study was to investigate the impact of radiation on different immune structures and immune cells in patients treated with CCRT followed by durvalumab.

Material And Methods: Clinicopathologic data, pre- and post-treatment blood counts, and dosimetric data were collected in patients treated with CCRT and durvalumab consolidation for LA-NSCLC.

RadioLigand Therapy with [Lu]Lu-PSMA-617 for Salivary Gland Cancers: Literature Review and First Compassionate Use in France.

Salivary gland cancers are rare tumors comprising a large group of heterogeneous tumors with variable prognosis. Their therapeutic management at a metastatic stage is challenging due to the lack of therapeutic lines and the toxicity of treatments. [Lu]Lu-PSMA-617 (prostate-specific membrane antigen) is a vectored radioligand therapy (RLT) initially developed to treat castration-resistant metastatic prostate cancer with encouraging results in terms of efficacy and toxicity.

Metatranscriptomics analysis reveals a novel transcriptional and translational landscape during Middle East respiratory syndrome coronavirus infection.

Among all RNA viruses, coronavirus RNA transcription is the most complex and involves a process termed "discontinuous transcription" that results in the production of a set of 3'-nested, co-terminal genomic and subgenomic RNAs during infection. While the expression of the classic canonical set of subgenomic RNAs depends on the recognition of a 6- to 7-nt transcription regulatory core sequence (TRS), here, we use deep sequence and metagenomics analysis strategies and show that the coronavirus transcriptome is even more vast and more complex than previously appreciated and involves the production of leader-containing transcripts that have canonical and noncanonical leader-body junctions. Moreover, by ribosome protection and proteomics analyses, we show that both positive- and negative-sense transcripts are translationally active.

Genome Sequences of Diverse Human Cytomegalovirus Strains with Utility in Drug Screening and Vaccine Evaluation.

Cytomegalovirus displays genetic heterogeneity, which has implications for antiviral and vaccine development. Many studies have focused on laboratory isolates that have been extensively adapted for growth on fibroblasts. Here, we report whole-genome sequences for 10 human cytomegalovirus (HCMV) strains that readily grow on ARPE-19 human retinal pigment epithelial cells.

Complete Genome Sequences of Mandrillus leucophaeus and Papio ursinus Cytomegaloviruses.

The complete genome sequences of Mandrillus leucophaeus and Papio ursinus cytomegaloviruses were determined. An isolate from a drill monkey, OCOM6-2, and an isolate from a chacma baboon, OCOM4-52, were subjected to pyrosequencing and assembled. Comparative alignment of published primate cytomegaloviruses (CMVs) showed variable sequence conservation between species.

A revised chronology for the Grotte Vaufrey (Dordogne, France) based on TT-OSL dating of sedimentary quartz.

Grotte Vaufrey, located in the Dordogne region of southwestern France, is well known for its substantial archaeological sequence containing a succession of Acheulean and Mousterian occupations. While over the last thirty years numerous studies have attempted to outline a detailed chronostratigraphy for this important sequence, the failure to employ a common chronological framework has complicated its interpretation. Here, we aim to resolve these inconsistencies by providing a new chronology for the site based on luminescence dating.

Neandertals made the first specialized bone tools in Europe.

Modern humans replaced Neandertals ∼40,000 y ago. Close to the time of replacement, Neandertals show behaviors similar to those of the modern humans arriving into Europe, including the use of specialized bone tools, body ornaments, and small blades. It is highly debated whether these modern behaviors developed before or as a result of contact with modern humans.

[Hemostasis task descriptive file meeting the standard NF EN ISO 15 189 requirements].

This work is a presentation of an hemostasis task descriptive file in regard with the requirements of the standard NF EN ISO 15189. The chosen method is based on the Deming wheel, that is to say: 1) Forward planning, done by the quality department of the work organization. 2) Drafting of a task descriptive file of the laboratory technician (working hours, equipments, done analysis, three levels of qualification, recurrent tasks, periodic hours, occasional hours, reference document).

Analysis of Aurignacian interstratification at the Chatelperronian-type site and implications for the behavioral modernity of Neandertals.

The Châtelperronian is a Neandertal-associated archeological culture featuring ornaments and decorated bone tools. It is often suggested that such symbolic items do not imply that Neandertals had modern cognition and stand instead for influences received from coeval, nearby early modern humans represented by the Aurignacian culture, whose precocity would be proven by stratigraphy and radiocarbon dates. The Grotte des Fées at Châtelperron (France) is the remaining case of such a potential Châtelperronian-Aurignacian contemporaneity, but reanalysis shows that its stratification is poor and unclear, the bone assemblage is carnivore-accumulated, the putative interstratified Aurignacian lens in level B4 is made up for the most part of Châtelperronian material, the upper part of the sequence is entirely disturbed, and the few Aurignacian items in levels B4-5 represent isolated intrusions into otherwise in situ Châtelperronian deposits.

[Prospective assessment of information programme for blood transfusion in critical care patients].

Objectives: Assessment of oral and written information programme for blood transfusion in critical care patients and study of factors associated with the biological follow-up.

Study Design: Prospective study in one intensive care unit.

Patients And Methods: All blood recipients in 2000 were orally informed of transfusion process.

Reversible myelofibrosis induced by tuberculosis.

Tuberculosis and myelofibrosis are reported in conjunction often enough to raise the possibility that a relationship exists between the 2 entities. However, whether tuberculosis stimulates a secondary fibrotic reaction or develops in patients who have preexisting myeloproliferative disorders is not clear. We describe the case of a 28-year-old man in whom myelofibrosis disappeared completely after administration of antituberculous treatment, which suggests that a causal relationship exists between the 2 disease entities.

[Surveillance and prognosis of "T1" superficial tumors of the bladder. Homogeneous series of 88 cases followed for 1 to 22 years].

Objective: Define the prognostic factors (risk of recurrence and risk of progression) and the rules for surveillance of stage T1 papillary bladder tumours based on the clinical course of a homogeneous patient series.

Material And Method: 88 T1 bladder tumours recruited from 1975 to 1995 and regularly followed by the same urologist. The follow-up ranged from 1 to 22 years with a mean of 52 months and a median of 48 months.

[Surveillance and prognosis of "Ta" superficial tumors of the bladder. Homogeneous series of 138 cases followed for 1 to 18 years].

Objective: To identify prognostic factors (risk of recurrence and risk of progression) and define the rules for the surveillance of stage Ta superficial bladder cancers, based on the follow-up of a homogeneous patient series.

Material And Method: 138 Ta bladder cancers were recruited from 1975 to 1995 and regularly followed by the same urologist. The follow-up was 1 to 18 years with a mean of 66 months and a median of 60 months.

Severe infection caused by Stomatococcus mucilaginosus in a neutropenic patient: case report and review of the literature.

A 24-year-old female, in neutropenic phase after chemotherapy for acute myelogenous leukemia (on day 15) was admitted in intensive care unit for infectious pneumonia. Two strains of Stomatococcus mucilaginosus were isolated from peripheral blood cultures. No microorganisms were yielded from bronchoalveolar lavage.

Elastase activity in cartilage extracts and synovial fluids from subjects with osteoarthritis or rheumatoid arthritis: the prominent role of metalloproteinases.

Objective: Polymorphonuclear leukocyte (PMN) elastase is able to degrade the extra-cellular matrix components of cartilage. However, in vitro several proteinases can degrade elastin. The purpose of this study was to evaluate the role of the serine proteinases and metalloproteinases in the elastase activity measured in cartilage extracts from patients with osteoarthritis (OA), as well as in synovial fluid (SF) from patients with rheumatoid arthritis (RA) and OA.

[Treatment of patients with atherosclerotic carotid stenosis in 1993. Indications and long-term results of surgery].

Cerebrovascular accidents (CVAs) are the third commonest cause of death in France. Approximately 15% of them are due to stenosis of the extracranial internal carotid. The fact that a third of CVAs are followed by death and another third by major handicaps leads to the need for careful prevention.

[A rare cause of portal vein thrombosis: closed abdominal trauma].

Abdominal trauma is a rare and poorly documented cause of portal vein thrombosis. We report here the case of a patient in whom portal vein thrombosis was diagnosed one month after an abdominal blunt trauma. Post-traumatic origin of thrombosis was confirmed by the negativity of an exhaustive aetiological investigation.

[Postoperative hydrocephalus. Automatic control of intracranial pressure in patients treated with external drainage].

An experimental analogical device is described; it is able to stabilize the intracranial pressure (ICP) at an adjustable value in subjects exhibiting hydrocephalus. The mean ICP is measured by the intraventricular catheter, using a pressure measurement apparatus of conventional electric design. The result and two practical examples are presented.