Identification of cellulolytic lactic acid bacteria from the intestines of laying hens given AKBISprob based on 16S ribosomal ribonucleic acid gene analysis.

Background And Aim: Supplementation of AKBISprob (developed in a previous study) in feed can improve production efficiency and poultry health, especially laying hens. In addition, it can also increase cellulolytic lactic acid bacteria (LAB) in chicken intestines, but these bacteria are still unknown; thus, they need to be identified. This study aimed to identify cellulolytic LAB in the intestines of laying hens administered AKBISprob based on 16S ribosomal ribonucleic acid (16S rRNA) gene analysis.

Cloning and expression of GRA-4 recombinant protein as a toxoplasmosis diagnostic kit candidate.

Aim: The objective of this study was to produce recombinant protein GRA-4 (rGRA-4) of a local isolate as a candidate for a toxoplasmosis diagnosis kit in BL21 (DE3) competent cells using pET SUMO plasmid.

Materials And Methods: Samples used were stock tachyzoites DNA from the Parasitology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta. Amplified GRA-4 polymerase chain reaction product of tachyzoite DNA was cloned in the pET-SUMO TA cloning vector.