Publications by authors named "Tetta Takahashi"
Article Synopsis
- The first-generation vector pCMViR-TSC shows significantly higher gene expression (10- to 100-fold) compared to standard plasmids but is limited to transient gene expression in mammalian cells.
- To address this limitation, a new second-generation vector, pSAKA-4B, is introduced, enabling stable integration of genes into mammalian DNA with high efficiency.
- This improved vector system allows for indefinite gene expression, making it valuable for basic research and potential clinical applications like gene therapy.
Background: Our earlier research revealed that the secreted lysyl oxidase-like 4 (LOXL4) that is highly elevated in triple-negative breast cancer (TNBC) acts as a catalyst to lock annexin A2 on the cell membrane surface, which accelerates invasive outgrowth of the cancer through the binding of integrin-β1 on the cell surface. However, whether this machinery is subject to the LOXL4-mediated intrusive regulation remains uncertain.
Methods: Cell invasion was assessed using a transwell-based assay, protein-protein interactions by an immunoprecipitation-Western blotting technique and immunocytochemistry, and plasmin activity in the cell membrane by gelatin zymography.