Tools that provide absolute quantification of biomolecules, particularly of proteins and their post-translational modifications, without needing suitable specific standards, are urgently demanded nowadays. To this end, we have significantly improved the recently introduced strategy based on CH addition to the plasma for absolute quantification of biomolecules using HPLC-ICP-MS. Addition of CO has been optimized and finally selected as a safer, more efficient quantitative strategy that is able to provide constant (<6% error) signal response factor for the six elements assayed (S, P, As, Se, Br, I) under compromised conditions.
View Article and Find Full Text PDFPurpose: The purpose of the present study was to obtain fundamental data for application of the cast-on method by evaluating the effect of mold temperatures on the interface between primary and secondary castings in detail.
Methods: Silver-palladium-gold alloy (Ag-Pd-Au), type-4 gold alloy (Type4), and chromium-cobalt alloy (Cr-Co) were used in the present study. A polished flat, square metal plate, 10.
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