Eco-friendly TEMPO/laccase/O biocatalytic system for degradation of Indigo Carmine: operative conditions and laccase inactivation.

The biocatalytic system laccase/TEMPO/O has attracted the attention of researchers over the past two decades. A variety of applications for the system include organic synthesis, modification of cellulose, and oxidative degradation of environmental contaminants. A rigorous and predictable quantitative assessment of the change in enzymatic activity under the influence of a mediator is important for such a system.