Publications by authors named "Tessa Van Royen"
The pandemic readiness toolbox needs to be extended, targeting different biomolecules, using orthogonal experimental set-ups. Here, we build on our Cov-MS effort using LC-MS, adding SISCAPA technology to enrich proteotypic peptides of the SARS-CoV-2 nucleocapsid (N) protein from trypsin-digested patient samples. The CovMS assay is compatible with most matrices including nasopharyngeal swabs, saliva, and plasma and has increased sensitivity into the attomole range, a 1000-fold improvement compared to direct detection in a matrix.
View Article and Find Full Text PDFHuman respiratory syncytial virus (RSV) is the leading cause of severe acute lower respiratory tract infections in infants worldwide. Nonstructural protein NS1 of RSV modulates the host innate immune response by acting as an antagonist of type I and type III interferon (IFN) production and signaling in multiple ways. Likely, NS1 performs this function by interacting with different host proteins.
View Article and Find Full Text PDFRespiratory syncytial virus (RSV) is the leading cause of severe acute lower respiratory tract infections in infants worldwide. Although several pattern recognition receptors (PRRs) can sense RSV-derived pathogen-associated molecular patterns (PAMPs), infection with RSV is typically associated with low to undetectable levels of type I interferons (IFNs). Multiple RSV proteins can hinder the host's innate immune response.
View Article and Find Full Text PDFObjectives: We report a reproducible automated radiosynthesis for large scale batch production of clinical grade Al[F]PSMA-11.
Methods: A SynthraFCHOL module was optimized to synthesize Al[F]PSMA-11 by Al[F]-chelation. Results Al[F]PSMA-11 was synthesized within 35min in a yield of 21 ± 3% (24.