Water-binding phospholipid nanodomains and phase-separated diacylglycerol nanodomains regulate enzyme reactions in lipid monolayers.

Phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) nanodomains covered with bound water as well as diacylglycerol 1-palmitoyl-2-oleoyl-sn-glycerol (POG) nanodomains separated from a lipid membrane were studied, using monolayer surfaces of POPC hydrolyzed by phospholipase C (PLC). The investigation was based on the analysis of compression isotherms and on atomic force microscope (AFM) observations of Langmuir-Blodgett (LB) films and Langmuir-Schaefer (LS) films. The results included reaction rate constants obtained by kinetic analysis of phosphocholine at surface pressures from 0.