Successful treatment of experimental murine vulvovaginal candidiasis with gentian violet.

This study evaluated the antifungal efficacy of gentian violet (GV) in an experimental vulvovaginal candidiasis (VVC) model. susceptibility and cytotoxicity assays were performed to validate the antifungal potential and safety of GV. The antifungal efficacy was then evaluated through comparative analysis of the fungal burden following treatment with GV or nystatin, as well as assessment of the vaginal tissue by histology and electron microscopy.

Analysis of the antifungal potential of extract against .

To investigate the antifungal potential of extracts against . Extracts were obtained as aqueous (EfraMat-22 and EfraMat-45) and methanolic/ethyl acetate fractions. Broth microdilution and disk diffusion assays showed that EfraMat-45 provided the best results in terms of minimum inhibitory concentration.

Polycaprolactone nanofibers colonized by HeLa cells: an alternative for investigation of fungal infection.

3D equivalent tissues can be used for studies of fungal infections. To develop 3D electrospun nanofibers using polycaprolactone (PCL) colonized by HeLa cells as a possible model for the investigation of fungal infection. A PCL solution was synthesized and electrospun.

Insight into the antifungals used to address human infection due to spp.: a scoping review.

Trichosporonosis infections have been increasing worldwide. Providing adequate treatment for these infections remains a challenge. This scoping review contains information about potential antifungals to treat this pathology.

Different expression levels of and genes contribute to recurrent vulvovaginal candidiasis by .

To study the behavior of in women with vulvovaginal candidiasis (VVC), recurrent VVC (RVVC) and asymptomatic (AS), regarding adhesion on HeLa cells and their ability to express secreted aspartic proteinases () genes, agglutinin-like sequence () genes and . The adhesion of to HeLa cells was evaluated by colony-forming units, and the expressed genes were evaluated by qRT-PCR. AS and VVC isolates showed greater ability to adhere HeLa cells when compared with RVVC isolate.

Impact of serial systemic infection on virulence factors.

To evaluate changes in virulence and pathogenicity approaches from after successive passages in a murine model of systemic candidiasis. Phenotypic assays were performed using colonies recovered from animals infected serially, totalizing five passages. A progressive infection was observed along the passages, with increased fungal burden and the presence of greater inflammatory areas in the histopathological findings.

Vulvovaginal candidiasis in a murine model of diabetes emphasizing the invasive ability of etiological agents.

To compare the pathogenesis of vulvovaginal candidiasis by three species in diabetic mice. Estrogenized and diabetic mice were challenged with , and . Diabetic animals infected with and maintained the highest fungal burden, despite of high levels of proinflammatory cytokines (IL-6 and TNF-α), respectively.

Silver nanoparticles stabilized with propolis show reduced toxicity and potential activity against fungal infections.

Elucidate the antifungal efficacy of biologically synthesized silver nanoparticles with ethanolic propolis extract (AgNPs PE) against the planktonic forms and biofilms of clinically important fungi. AgNPs were synthesized, characterized and evaluated for cytotoxicity, mutagenicity and antimicrobial activity. AgNPs PE displayed a colloidal appearance, good stability and size of 2.

Copolymeric micelles as efficient inert nanocarrier for hypericin in the photodynamic inactivation of species.

To evaluate the efficacy of photodynamic inactivation (PDI) mediated by hypericin encapsulated in P-123 copolymeric micelles (P123-Hyp) alone and in combination with fluconazole (FLU) against planktonic cells and biofilm formation of species PDI was performed using P123-Hyp and an LED device with irradiance of 3.0 mW/cm . Most of isolates (70%) were completely inhibited with concentrations up to 2.

Fusarium oxysporum is an onychomycosis etiopathogenic agent.

Aim: To evaluate and characterize the etiopathogenesis of the fusarial onychomycosis in an ex vivo study through fragments of sterile human nail, without the addition of any nutritional source.

Materials & Methods: The infection and invasion of Fusarium oxysporum in the nail were evaluated by scanning electron microscopy (SEM), CFU, matrix, histopathology and Fourier Transform Infrared Spectrometer coupled to an equipment with diamond accessory (FTIR-ATR).

Results: F.

γ-irradiation from radiotherapy improves the virulence potential of Candida tropicalis.

Aim: To evaluate if radiation used in radiotherapy can cause changes in the virulence potential of Candida tropicalis ATCC 750.

Materials & Methods: C. tropicalis was exposed in vitro to identical dose and scheme of irradiation would be used in patients with head and neck cancer.

A new small-molecule KRE2 inhibitor against invasive Candida parapsilosis infection.

Aim: To investigate the antifungal activity of MOL3, a small molecule that was selected by virtual screening, against Candida spp.

Materials & Methods: The antifungal activity of MOL3 was evaluated using standard strains and clinical isolates. Activity was evaluated in both in vitro tests and animal models.

Propolis: a potential natural product to fight Candida species infections.

Aim: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms.

Materials & Methods: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs.

Animal models for the effective development of atrophic vaginitis therapies: possibilities and limitations.

Introduction: Vaginal atrophy (VA) is an inflammation of the vagina that develops when there is a significant decrease in levels of the estrogen. Prolonged periods of hypoestrogenism may induce severe VA and treatment is essential. This is a significant problem which requires more focused attention for the development of existing and future therapies.