Publications by authors named "Terezie Prilepskaja"
Article Synopsis
- The formation of the 43S pre-initiation complex (PIC) is crucial for canonical mRNA translation in eukaryotes, involving initiator Met-tRNA and several eukaryotic initiation factors (eIFs) bound to the small ribosomal subunit (40S).
- Structural differences in the 40S ribosomal subunit of trypanosomatids, like Trypanosoma cruzi (the cause of Chagas disease), indicate variability in translation initiation compared to mammals.
- The study reveals unique features of the 43S PIC structure, including a distinct eIF3 structure, interactions with rRNA expansion segments, and the role of a kinetoplastid-specific helicase, supported by biochemical assays and mass spect
One of the key roles of the 12-subunit eukaryotic translation initiation factor 3 (eIF3) is to promote the formation of the 43S and 48S pre-initiation complexes (PICs). However, particular contributions of its individual subunits to these two critical initiation reactions remained obscure. Here, we adapted formaldehyde gradient cross-linking protocol to translation studies and investigated the efficiency of the 43S and 48S PIC assembly in knockdowns of individual subunits of human eIF3 known to produce various partial subcomplexes.
View Article and Find Full Text PDFThe mechanism used by mouse polyomavirus (MPyV) overcomes the crowded cytosol to reach the nucleus has not been fully elucidated. Here, we investigated the involvement of importin α/β1 mediated transport in the delivery of MPyV genomes into the nucleus. Interactions of the virus with importin β1 were studied by co-immunoprecipitation and proximity ligation assay.
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