Publications by authors named "Tereza Vackova"
Background: Manufacturers and diagnostic companies often recommend on-site verification of analytical performance in the clinical laboratory. The validation process used by manufacturers is rarely described in detail, and certain information on analytical performance is missing from the product sheet, especially for immunoanalytical methods. We describe an approach to the detailed validation of an ELISA method for the measurement of proprotein convertase subtilisin/kexin type 9 (PCSK9) plasma concentrations.
View Article and Find Full Text PDFBackground: Prothrombin/Protein Induced by Vitamin K Absence-II (PIVKA-II) is a candidate biomarker of hepatocellular cancer, recommended both for diagnostics and monitoring. The aim was to evaluate biological variation (BV) of serum PIVKA-II.
Methods: Within-subject (CV) and between-subject (CV) BV estimates were assessed in 14 healthy volunteers in a 6-week protocol.
Article Synopsis
- * Mites prefer the upper layers of mattresses for feeding, where temperatures range from 32-36 °C and humidity is between 55-59%, while non-feeding mites dwell deeper.
- * Laboratory analyses showed that the optimal growth for mite populations occurs at about 28 °C and 85% RH, but respiration rates display two peaks at 30 °C, indicating a discrepancy that requires further investigation.
House dust mites inhabit bed mattresses contaminating them with allergens. A strong temperature/moisture gradient exists in mattresses when it is used by humans daily. Here, we studied migration patterns of the mite Dermatophagoides farinae in continuous and time-discontinuous temperature gradients consisting of five sectors with 19-23, 23-28, 28-32, 32-36 and 36-41 °C, containing dye-labeled diets as an indicator of mite presence and feeding.
View Article and Find Full Text PDFBackground: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is involved in the regulation of LDL receptors. Inhibition of PCSK9 increase uptake of LDL-particles and pathogen-associated molecular patterns (PAMPs). The aim of our study was to evaluate biological variation of serum PCSK9.
View Article and Find Full Text PDFBackground: Fibroblast growth factor 23 (FGF23), a potent regulator of phosphate and vitamin D metabolism, is a new biomarker of kidney, bone and cardiovascular disorders. The aim of this study was to assess the biological variation of intact fibroblast growth factor 23 (iFGF23).
Methods: The within-subject (CV) and between-subject (CV) biological variations were assessed in 14 healthy volunteers in a six-week protocol (seven samples).