Molecular Cloning and Characterization of Protein Kinase C from the Sea Urchin Lytechinus pictus: (protein kinase C/cDNA cloning/polyclonal antibodies/developmental expression).

Protein kinase C (PKC) has been shown to play a role in events involved in fertilization such as activation of the Na /H antiporter and an NADPH dependent oxidase. In addition, it is involved in cell fate programming later in development of the sea urchin embryo. In order to further address the role of PKC in sea urchin development, we have screened a Lytechinus pictus ovary tissue cDNA library and identified one clone for sea urchin protein kinase C (suPKC1).