To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Exosomes were isolated from HEK293T cells and co-incubated with boar sperm . Internalized exosomes were detected within 10 min of co-incubation.
View Article and Find Full Text PDFObjective: To study how temperature, serum, and gonadotropin supplementation affect the organotypic culture of human immature testicular tissue (ITT) in vitro.
Design: Experimental basic science study.
Setting: Reproductive biology laboratory.
As gonadotoxic adverse effects of antineoplastic treatments can result in infertility, gamete cryopreservation is routinely offered to patients as the strategy to preserve their fertility. However, there are many cases where gold standards cannot be applied, as is the case for prepubertal cancer patients and others unable to produce gametes or their precursors at the moment of diagnosis. With an increasing number of cancer survivors in our society, strategies using either cryopreserved gonadal tissue or stem cells have been developed to allow cancer survivors to achieve fatherhood, and recent advances in the field have increased public interest.
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