N-halamine incorporated antimicrobial nonwoven fabrics for use against avian influenza virus.

Airborne pathogens are one of the most common avenues leading to poultry diseases. Preventing the avian influenza (AI) virus from entering the chicken hatchery house is critical for reducing the spread and transmission of AI disease. Many studies have investigated the incorporation of antimicrobials into air filters to prevent viruses from entering the indoor environment.

Detection and isolation of infectious laryngotracheitis virus on a broiler farm after a disease outbreak.

A broiler farm in North Alabama suffered a mild infectious laryngotracheitis (ILT) outbreak, as determined by clinical disease and PCR. The poultry integrator sought help to control further outbreaks in subsequent flocks. Samples were collected from various areas of the poultry houses on the farm over an 8-wk period.

Development of TaqMan real-time RT-PCR for detection of avian reoviruses.

Avian reoviruses (ARVs) are an important cause of economic losses in commercial poultry. A TaqMan real-time RT-PCR assay for detecting of ARVs was developed. The primer-probe set was from the conserved region of ARV S4 genome segment.

Evaluation of the immune response and detection of infectious bursal disease viruses by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay after in ovo vaccination of commercial broilers.

Maternal immunity can interfere with infectious bursal disease virus (IBDV) vaccine efficacy. The effect of maternal antibody (MacAb) on the immune response and detection of two vaccine viruses, an IBDV immune complex (cx) and IBDV-2512, was investigated. Vaccines were administered in ovo at 100 mean embryo infectious dose to commerical broiler embryos derived from young (29 wk) or old (63 wk) flocks.