Proficiency Barrier in Track and Field: Adaptation and Generalization Processes.

The literature on motor development and training assumes a hierarchy for learning skills-learning the "fundamentals"-that has yet to be empirically demonstrated. The present study addressed this issue by verifying (1) whether this strong hierarchy (i.e.

Blastic Plasmacytoid Dendritic Cell Neoplasm: A Rare Entity in Clinical Practice.

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is an exceedingly rare and aggressive hematologic malignancy. In the current World Health Organization classification, it is classified among histiocytic/dendritic cell neoplasms. This report describes the case of an 85-year-old female with a complex medical history, including rheumatoid arthritis, who presented with a one-month history of low-grade fever, anorexia, and unexplained weight loss.

Development and validation of a quick, automated, and reproducible ATR FT-IR spectroscopy machine-learning model for typing.

The reliability of Fourier-transform infrared (FT-IR) spectroscopy for typing and outbreak control has been previously assessed, but issues remain in standardization and reproducibility. We developed and validated a reproducible FT-IR with attenuated total reflectance (ATR) workflow for the identification of lineages. We used 293 isolates representing multidrug-resistant lineages causing outbreaks worldwide (2002-2021) to train a random forest classification (RF) model based on capsular (KL)-type discrimination.

sp. nov. (formerly genomic species 3) and sp. nov. (formerly genomic species 8) isolated from female urinary microbiome.

During an ongoing female urinary microbiome research study, strains c17Ua_112 and c31Ua_26 isolated from urine samples of a patient diagnosed with overactive bladder and a healthy postmenopausal woman, respectively, could not be allocated to any species with valid names. In this work, we aimed to characterize these strains. The 16S rRNA gene sequences confirmed that these strains are members of the genus .

Diploid Nuclei Occur throughout the Life Cycles of Pucciniales Fungi.

Within Eukaryotes, fungi are the typical representatives of haplontic life cycles. Basidiomycota fungi are dikaryotic in extensive parts of their life cycle, but diploid nuclei are known to form only in basidia. Among Basidiomycota, the Pucciniales are notorious for presenting the most complex life cycles, with high host specialization, and for their expanded genomes.

sp. nov., isolated from a soil sample.

A Gram-stain-negative strain, designated BR102, isolated from a soil sample in Brazil was characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that strain BR102 belonged to the genus . The - and whole-genome-based phylogeny, and multilocus sequence analysis based on concatenated partial , , and sequences strongly supported a clade encompassing strain BR102 and a strain from public database that was distinct from currently recognized species of the genus .

Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New Species.

The genus is frequently found in the female urinary microbiome (FUM). In-depth characterization of at the species level has been barely exploited. During ongoing FUM research studies, eight strains (c8Ua_144, c8Ua_172, c8Ua_174, c8Ua_181, c9Ua_112, c19Ua_109, c19Ua_121, and c21Ua_68) isolated from urine samples of healthy women or diagnosed with overactive bladder could not be allocated to any valid species.

Urinary Microbiome of Reproductive-Age Asymptomatic European Women.

The knowledge of bacterial species diversity within the female urinary microbiome (FUM) is essential for understanding the role of the FUM in urinary tract health and disease. This study aimed to characterize the bacterial species diversity of the FUM of asymptomatic reproductive-age European women by combining extended culturomics and long-read sequencing of the near-full-length 16S rRNA gene. A total of 297 bacterial species (median of 53 species/sample) were identified, yet only 22% of the species were detected by both culture and sequencing methods.

Genomic diversity of genus .

The genus (formerly ) contains multiple species considered to be adapted to vertebrates, yet their genomic diversity has not been explored. In this study, we performed comparative genomic analysis of (22 species; 332 genomes) isolated from different niches, further focusing on human strains (11 species; 74 genomes) and their adaptation features to specific body sites. Phylogenomic analysis of showed misidentification of some strains deposited in public databases and existence of putative novel species.

Acute organ failure and risk of admission to intensive medical care in cancer patients: a single center prospective cohort study.

Objective: To ascertain the cumulative incidence of acute organ failure and intensive care unit admission in cancer patients.

Methods: This was a single-center prospective cohort study of adult cancer patients admitted for unscheduled inpatient care while on systemic cancer treatment.

Results: Between August 2018 and February 2019, 10,392 patients were on systemic treatment, 358 had unscheduled inpatient care and were eligible for inclusion, and 285 were included.

Evidence on the contribution of community gardens to promote physical and mental health and well-being of non-institutionalized individuals: A systematic review.

Introduction: There has been growing interest in community gardens as an effective and affordable health promotion strategy. However, most available evidence is derived from qualitative studies, whereas quantitative research on this subject is limited.

Objectives: To synthetize the literature about physical and mental health outcomes associated with community gardening.

The Impact of Writing About Gratitude on the Intention to Engage in Prosocial Behaviors During the COVID-19 Outbreak.

The ongoing coronavirus disease 2019 (COVID-19) pandemic has quickly swept the globe leaving a devastating trail of lost human lives and leading to a public health and economic crisis. With this in mind, prosociality has been heralded as a potential important factor to overcome the negative effects of the pandemic. As such, in this study, we examined the effectiveness of a brief reflexive writing exercise about recent experiences of gratitude on individuals' intentions to engage in prosocial behaviors using a sample of 253 participants living in Portugal and 280 participants living in Brazil.

sp. nov. and sp. nov. isolated from urine of healthy women.

Two Gram-stain-positive strains, c9Ua_26_M and c11Ua_112_M, were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus . Phylogenetic analysis based on gene sequences and core genomes showed that each strain formed a separated branch and are closest to DSM 5837.

MicroMundo@UPorto: an experimental microbiology project fostering student's antimicrobial resistance awareness and personal and social development.

Antimicrobial resistance (AMR) is a global societal challenge requiring the contribution of professionals along with general community citizens for their containment. Portugal is one of the European countries where a lack of knowledge on the correct use of antimicrobials and AMR problematic is preeminent. Moreover, youth demotivation to pursue science careers is emerging.

Citrobacter telavivum sp. nov. with chromosomal mcr-9 from hospitalized patients.

Strains 6105 and 6106, recovered from colonized patients in a hospital in Tel-Aviv, Israel, were compared with currently known species of the genus Citrobacter by a polyphasic taxonomic approach. Strains were characterized by whole-genome sequencing, 16S rRNA and recN gene sequencing, multilocus sequence analysis (MLSA), average nucleotide identity (ANI), Genome-to-Genome Distance Calculator (GGDC), and biochemical tests. The location and genetic surrounding of antibiotic resistance genes were investigated, and antibiotic susceptibility profiles were determined by broth microdilution or agar dilution methods.

The status of the species Dicks . 2000. Request for an opinion.

During a recent study on members of the genus we realized that cultures of TV 1018 (=DSM 13171=ATCC 700934) are no longer available from the online catalogue of the German Collection of Microorganisms and Cell Cultures GmbH, being displayed as at the American Type Culture Collection. Based on data currently available, the organism deposited as ATCC 700934 is a member of the species subs. .

sp. nov., a new species of group.

One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161M, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161M belonged to the genus . Phylogenetic analysis based on and gene sequences strongly supported a clade encompassing strains c10Ua161M and eight other strains from public databases, distinct from currently recognized species of the genus Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.

Citrobacter portucalensis sp. nov., isolated from an aquatic sample.

A Gram-stain-negative strain, A60T, isolated from a water well sample in Portugal, was characterized phenotypically, genotypically and phylogenetically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A60T belonged to the genus Citrobacter, and recN gene phylogeny revealed one strongly supported clade encompassing strain A60T and 13 other strains from public databases, distinct from currently recognized species of the genus Citrobacter. Furthermore, multilocus sequence analysis (MLSA) based on concatenated partial fusA, leuS, pyrG and rpoB sequences confirmed the classification obtained with the recN sequence.

Autosomal SNPs study of a population sample from Southern Portugal and from a sample of immigrants from Guinea-Bissau residing in Portugal.

In recent years, autosomal single nucleotide polymorphisms (SNPs) have been comprehensively investigated in forensic research due to their usefulness in certain circumstances in complementing short tandem repeats (STRs) analysis, or even for use on their own when analysis of STRs fails. However, as with STRs, in order to properly use SNP markers in forensic casuistic we need to understand the population and forensic parameters in question. As a result of Portugal's colonial history during the time of empire, and the subsequent process of decolonization, some African individuals migrated to Portugal, giving rise to large African and African-descendent communities.

Citrobacter europaeus sp. nov., isolated from water and human faecal samples.

Strains 97/79T and A121, recovered respectively from human faeces and well water, were compared to currently known species of the genus Citrobacter using genotypic and phenotypic approaches. Multilocus sequence analysis based on housekeeping genes fusA, leuS, pyrG, rpoB and recN, showed that the two strains formed a distinct phylogenetic lineage within the genus Citrobacter. Average nucleotide identity (ANI) between strains 97/79T and A121 was 99.

Characterization of GlobalFiler loci in Angolan and Guinean populations inhabiting Southern Portugal.

We analyzed the GlobalFiler short tandem repeat (STR) loci for 152 and 70 unrelated individuals from Angolan and Guinean immigrant populations inhabiting Southern Portugal, respectively. After Bonferroni correction, no significant deviations from the Hardy-Weinberg equilibrium and linkage disequilibrium were detected for either population. For the Angolan population, SE33 was the most informative marker.

Study of InDel genetic markers with forensic and ancestry informative interest in PALOP's immigrant populations in Lisboa.

The migratory phenomenon in Portugal has become one of the main factors for the genetic variability. In the last few years, a new class of autosomal insertion/deletion markers-InDel-has attracted interest in forensic genetics. Since there is no data for InDel markers of Portuguese-speaking African countries (PALOP) immigrants living in Lisboa, our aim is the characterization of those groups of individuals by typing them with at least 30 InDel markers and to compare different groups of individuals/populations.