Pellet cryopreservation for chicken semen: effects of sperm working concentration, cryoprotectant concentration, and equilibration time during in vitro processing.

The aim of the study was to standardize the pellet cryopreservation procedure for chicken semen. Mericanel della Brianza male chicken breeders (Italian breed) were used. Pooled semen samples were processed according to the following conditions: (1) dilution in prefreezing extender to 1 versus 1.