SSR markers for subg. (Gnaphalieae: Asteraceae) and cross-amplification in three other subgenera.

Premise Of The Study: Microsatellite primers were developed for the first time in the genus (Gnaphalieae: Asteraceae). These markers will facilitate low-scale phylogenetic, phylogeographic, and population genetic studies within the genus .

Methods And Results: Ten pairs of polymorphic microsatellite primers (as well as five pairs of monomorphic primers) were identified and optimized on two species of ( and ) using a microsatellite-enrichment library method and 454 GS-FLX technique.

Microsatellite marker development for the tetraploid (Plantaginaceae) using next-generation sequencing and high-resolution melting analyses.

Premise Of The Study: The tetraploid (Plantaginaceae) is a narrow endemic to the Iberian Peninsula. Specific microsatellite markers were developed to investigate genetic structure and diversity.

Methods And Results: A total of 15 polymorphic markers were characterized on three populations of , using a microsatellite-enriched library on an Ion Torrent sequencer and high-resolution melting (HRM) analyses to rapidly discard nonreliable, multicopy, and/or monomorphic loci.